20854

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rlegTA/AbiEii-AbiEi
Location 2085285..2086815 Replicon chromosome
Accession NC_004463
Organism Bradyrhizobium diazoefficiens USDA 110

Toxin (Protein)


Gene name rlegT Uniprot ID A0A562QN92
Locus tag BJA_RS09560 Protein ID WP_018647871.1
Coordinates 2085285..2086205 (-) Length 307 a.a.

Antitoxin (Protein)


Gene name rlegA Uniprot ID A0A810CIT3
Locus tag BJA_RS09565 Protein ID WP_026312796.1
Coordinates 2086195..2086815 (-) Length 207 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BJA_RS09545 (bll1924) 2081260..2081778 - 519 Protein_1886 exonuclease subunit SbcD -
BJA_RS09550 (bll1925) 2081787..2083895 - 2109 WP_011084726.1 DUF87 domain-containing protein -
BJA_RS09555 (bll1926) 2083903..2085207 - 1305 WP_011084727.1 SIR2 family protein -
BJA_RS09560 (bll1927) 2085285..2086205 - 921 WP_018647871.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
BJA_RS09565 (bll1928) 2086195..2086815 - 621 WP_026312796.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
BJA_RS09570 (bll1929) 2087029..2087775 - 747 WP_018647872.1 DCL family protein -
BJA_RS09575 (bll1930) 2087750..2090260 - 2511 WP_011084731.1 AAA family ATPase -
BJA_RS09580 2090223..2090450 - 228 WP_131234255.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 2049053..2310150 261097


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-264)

Antitoxin

Transcriptional regulator, AbiEi antitoxin

(12-56)


Sequences

Toxin        


Download         Length: 307 a.a.        Molecular weight: 34027.14 Da        Isoelectric Point: 5.2727

>T20854 WP_018647871.1 NC_004463:c2086205-2085285 [Bradyrhizobium diazoefficiens USDA 110]
MAREPRNIGASVRARLLDRARAERSDFQILLTRYALERLLYRLSASPHRDRFILKGAMLFVTWIADPFRPTRDLDLLGHG
DRDTEAIAETFRAICAQPVVDDGVTFDVAALMATPIREELEYGGVRVRTTATIAGARIPIQVDIGFGDAITPAAVEIDYP
TLLDAPTPHLRAYPVETVVAEKFAALVTLGVANSRLKDFYDLWVISRTFELRRAALAEAIQRTFERRGTVLPSVVPVGLT
DEFAETWAAQWRAFLERDRMAAAPDAFAVTVADLRLFLMPLVVGLKEERIWPPSGPWSPGAAIDEA

Download         Length: 921 bp

>T20854 NC_004463:c2086205-2085285 [Bradyrhizobium diazoefficiens USDA 110]
ATGGCGCGTGAGCCTCGCAACATCGGCGCATCAGTGCGAGCGCGGCTCCTCGATCGGGCACGTGCCGAGCGATCGGACTT
CCAGATTCTCCTGACGCGATATGCGCTCGAGCGTCTGCTCTACCGGCTAAGCGCGTCGCCGCACCGCGACCGCTTCATCC
TCAAAGGTGCGATGCTCTTCGTGACCTGGATAGCCGATCCGTTTCGGCCTACGCGCGATCTGGATCTGCTTGGCCACGGC
GACCGCGACACCGAAGCCATTGCCGAAACCTTCCGGGCCATTTGTGCGCAGCCGGTCGTCGATGACGGTGTGACGTTTGA
TGTCGCAGCGCTGATGGCGACCCCGATTCGTGAGGAGCTGGAATATGGTGGCGTGCGGGTCCGCACAACGGCAACTATCG
CCGGGGCGCGTATTCCGATCCAGGTCGACATCGGTTTCGGCGACGCCATTACACCCGCGGCGGTCGAGATCGACTATCCC
ACCCTGCTGGACGCCCCAACGCCGCACCTTCGCGCATATCCGGTCGAAACAGTGGTAGCTGAGAAATTCGCAGCGCTCGT
GACGCTCGGCGTGGCCAACAGTCGGCTCAAGGATTTCTATGATCTCTGGGTAATCTCCCGAACATTCGAGCTTCGCCGGG
CCGCTCTTGCCGAGGCAATTCAGCGGACCTTCGAGCGACGCGGAACGGTCCTCCCATCCGTTGTCCCGGTCGGTCTGACC
GATGAATTCGCGGAAACATGGGCCGCCCAGTGGCGCGCCTTCCTGGAGCGCGATCGGATGGCAGCCGCACCGGATGCTTT
TGCCGTTACCGTCGCCGATCTCCGCCTCTTCTTGATGCCCCTGGTCGTCGGATTGAAAGAAGAACGAATTTGGCCGCCGA
GCGGGCCATGGTCGCCGGGAGCAGCAATCGATGAGGCATAG

Antitoxin


Download         Length: 207 a.a.        Molecular weight: 23385.28 Da        Isoelectric Point: 10.0691

>AT20854 WP_026312796.1 NC_004463:c2086815-2086195 [Bradyrhizobium diazoefficiens USDA 110]
MPSTSAQRLRALDLLKTRGMLRLKDFIAEGIGPETLARLVREEAVVRPARGLYQLPDAHLEAAHTLAEAAVLVPKGVVCL
TSALQYHELTLQMPSAVWMAIDRSAWRPKIDYPPIRFVRFTGSALTEGIERHRIENVDVPITDPARTIVDCFRYRTKIGL
DVAMEGLREGLRRRRCKPDQLWHYARKARVWSVMRPYVEAMVSDGA

Download         Length: 621 bp

>AT20854 NC_004463:c2086815-2086195 [Bradyrhizobium diazoefficiens USDA 110]
ATGCCGTCCACCAGCGCCCAGCGACTTCGCGCGCTCGACCTCCTGAAGACCCGAGGAATGCTCCGGCTGAAGGATTTCAT
CGCCGAAGGCATCGGCCCGGAGACGCTTGCCCGTCTTGTTCGGGAGGAGGCAGTCGTCCGCCCAGCACGTGGGCTCTATC
AACTCCCCGACGCCCACCTCGAGGCTGCCCATACCCTCGCCGAGGCAGCAGTTCTGGTTCCAAAGGGTGTGGTCTGTCTT
ACCTCGGCCCTCCAATACCACGAACTGACGCTGCAGATGCCGTCGGCTGTATGGATGGCGATCGATCGCTCCGCCTGGCG
ACCCAAGATCGATTATCCACCTATCCGGTTCGTGCGATTCACCGGATCGGCGCTTACCGAAGGGATCGAGCGTCACCGCA
TTGAGAATGTCGACGTTCCTATCACGGATCCGGCGAGGACAATTGTCGATTGCTTTCGCTACCGAACCAAGATCGGCCTT
GACGTCGCGATGGAGGGGCTGCGCGAAGGCCTCCGCCGTCGCCGATGCAAGCCGGATCAACTCTGGCATTACGCTCGCAA
GGCGCGGGTCTGGTCGGTCATGCGTCCCTACGTCGAAGCAATGGTGTCCGATGGCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A562QN92

Antitoxin

Source ID Structure
AlphaFold DB A0A810CIT3

References