54148

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rlegTA/AbiEii-AbiEi
Location 1404674..1406204 Replicon chromosome
Accession NZ_CP011360
Organism Bradyrhizobium diazoefficiens USDA 110

Toxin (Protein)


Gene name rlegT Uniprot ID A0A562QN92
Locus tag AAV28_RS06240 Protein ID WP_018647871.1
Coordinates 1404674..1405594 (-) Length 307 a.a.

Antitoxin (Protein)


Gene name rlegA Uniprot ID A0A810CIT3
Locus tag AAV28_RS06245 Protein ID WP_026312796.1
Coordinates 1405584..1406204 (-) Length 207 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AAV28_RS42540 1400649..1401167 - 519 Protein_1254 exonuclease subunit SbcD -
AAV28_RS06230 1401176..1403284 - 2109 WP_011084726.1 DUF87 domain-containing protein -
AAV28_RS06235 1403292..1404596 - 1305 WP_011084727.1 SIR2 family protein -
AAV28_RS06240 1404674..1405594 - 921 WP_018647871.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
AAV28_RS06245 1405584..1406204 - 621 WP_026312796.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
AAV28_RS06250 1406418..1407164 - 747 WP_018647872.1 DCL family protein -
AAV28_RS06255 1407139..1409649 - 2511 WP_011084731.1 AAA family ATPase -
AAV28_RS45760 1409612..1409839 - 228 WP_131234255.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Integrative and Conjugative Element - - 1368442..1629539 261097


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-264)

Antitoxin

Transcriptional regulator, AbiEi antitoxin

(12-56)


Sequences

Toxin        


Download         Length: 307 a.a.        Molecular weight: 34027.14 Da        Isoelectric Point: 5.2727

>T54148 WP_018647871.1 NZ_CP011360:c1405594-1404674 [Bradyrhizobium diazoefficiens USDA 110]
MAREPRNIGASVRARLLDRARAERSDFQILLTRYALERLLYRLSASPHRDRFILKGAMLFVTWIADPFRPTRDLDLLGHG
DRDTEAIAETFRAICAQPVVDDGVTFDVAALMATPIREELEYGGVRVRTTATIAGARIPIQVDIGFGDAITPAAVEIDYP
TLLDAPTPHLRAYPVETVVAEKFAALVTLGVANSRLKDFYDLWVISRTFELRRAALAEAIQRTFERRGTVLPSVVPVGLT
DEFAETWAAQWRAFLERDRMAAAPDAFAVTVADLRLFLMPLVVGLKEERIWPPSGPWSPGAAIDEA

Download         Length: 921 bp

>T54148 NZ_CP011360:c1405594-1404674 [Bradyrhizobium diazoefficiens USDA 110]
ATGGCGCGTGAGCCTCGCAACATCGGCGCATCAGTGCGAGCGCGGCTCCTCGATCGGGCACGTGCCGAGCGATCGGACTT
CCAGATTCTCCTGACGCGATATGCGCTCGAGCGTCTGCTCTACCGGCTAAGCGCGTCGCCGCACCGCGACCGCTTCATCC
TCAAAGGTGCGATGCTCTTCGTGACCTGGATAGCCGATCCGTTTCGGCCTACGCGCGATCTGGATCTGCTTGGCCACGGC
GACCGCGACACCGAAGCCATTGCCGAAACCTTCCGGGCCATTTGTGCGCAGCCGGTCGTCGATGACGGTGTGACGTTTGA
TGTCGCAGCGCTGATGGCGACCCCGATTCGTGAGGAGCTGGAATATGGTGGCGTGCGGGTCCGCACAACGGCAACTATCG
CCGGGGCGCGTATTCCGATCCAGGTCGACATCGGTTTCGGCGACGCCATTACACCCGCGGCGGTCGAGATCGACTATCCC
ACCCTGCTGGACGCCCCAACGCCGCACCTTCGCGCATATCCGGTCGAAACAGTGGTAGCTGAGAAATTCGCAGCGCTCGT
GACGCTCGGCGTGGCCAACAGTCGGCTCAAGGATTTCTATGATCTCTGGGTAATCTCCCGAACATTCGAGCTTCGCCGGG
CCGCTCTTGCCGAGGCAATTCAGCGGACCTTCGAGCGACGCGGAACGGTCCTCCCATCCGTTGTCCCGGTCGGTCTGACC
GATGAATTCGCGGAAACATGGGCCGCCCAGTGGCGCGCCTTCCTGGAGCGCGATCGGATGGCAGCCGCACCGGATGCTTT
TGCCGTTACCGTCGCCGATCTCCGCCTCTTCTTGATGCCCCTGGTCGTCGGATTGAAAGAAGAACGAATTTGGCCGCCGA
GCGGGCCATGGTCGCCGGGAGCAGCAATCGATGAGGCATAG

Antitoxin


Download         Length: 207 a.a.        Molecular weight: 23385.28 Da        Isoelectric Point: 10.0691

>AT54148 WP_026312796.1 NZ_CP011360:c1406204-1405584 [Bradyrhizobium diazoefficiens USDA 110]
MPSTSAQRLRALDLLKTRGMLRLKDFIAEGIGPETLARLVREEAVVRPARGLYQLPDAHLEAAHTLAEAAVLVPKGVVCL
TSALQYHELTLQMPSAVWMAIDRSAWRPKIDYPPIRFVRFTGSALTEGIERHRIENVDVPITDPARTIVDCFRYRTKIGL
DVAMEGLREGLRRRRCKPDQLWHYARKARVWSVMRPYVEAMVSDGA

Download         Length: 621 bp

>AT54148 NZ_CP011360:c1406204-1405584 [Bradyrhizobium diazoefficiens USDA 110]
ATGCCGTCCACCAGCGCCCAGCGACTTCGCGCGCTCGACCTCCTGAAGACCCGAGGAATGCTCCGGCTGAAGGATTTCAT
CGCCGAAGGCATCGGCCCGGAGACGCTTGCCCGTCTTGTTCGGGAGGAGGCAGTCGTCCGCCCAGCACGTGGGCTCTATC
AACTCCCCGACGCCCACCTCGAGGCTGCCCATACCCTCGCCGAGGCAGCAGTTCTGGTTCCAAAGGGTGTGGTCTGTCTT
ACCTCGGCCCTCCAATACCACGAACTGACGCTGCAGATGCCGTCGGCTGTATGGATGGCGATCGATCGCTCCGCCTGGCG
ACCCAAGATCGATTATCCACCTATCCGGTTCGTGCGATTCACCGGATCGGCGCTTACCGAAGGGATCGAGCGTCACCGCA
TTGAGAATGTCGACGTTCCTATCACGGATCCGGCGAGGACAATTGTCGATTGCTTTCGCTACCGAACCAAGATCGGCCTT
GACGTCGCGATGGAGGGGCTGCGCGAAGGCCTCCGCCGTCGCCGATGCAAGCCGGATCAACTCTGGCATTACGCTCGCAA
GGCGCGGGTCTGGTCGGTCATGCGTCCCTACGTCGAAGCAATGGTGTCCGATGGCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A562QN92

Antitoxin

Source ID Structure
AlphaFold DB A0A810CIT3

References