Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1690546..1691158 Replicon chromosome
Accession NZ_CP076594
Organism Erwinia amylovora isolate CP20086202

Toxin (Protein)


Gene name hicA Uniprot ID D2TUP9
Locus tag KQH36_RS07550 Protein ID WP_012906750.1
Coordinates 1690979..1691158 (-) Length 60 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID D4I356
Locus tag KQH36_RS07545 Protein ID WP_004157632.1
Coordinates 1690546..1690956 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQH36_RS07515 (KQH36_07530) 1686204..1686818 - 615 WP_004166850.1 DapH/DapD/GlmU-related protein -
KQH36_RS07520 (KQH36_07535) 1687424..1687696 + 273 WP_004157637.1 hypothetical protein -
KQH36_RS07525 (KQH36_07540) 1687707..1687880 + 174 WP_013036045.1 hypothetical protein -
KQH36_RS07530 (KQH36_07545) 1687975..1688148 + 174 WP_004157636.1 hypothetical protein -
KQH36_RS07535 (KQH36_07550) 1688406..1688618 + 213 WP_004157635.1 hypothetical protein -
KQH36_RS07540 (KQH36_07555) 1689489..1690490 - 1002 WP_004157633.1 acyltransferase -
KQH36_RS07545 (KQH36_07560) 1690546..1690956 - 411 WP_004157632.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KQH36_RS07550 (KQH36_07565) 1690979..1691158 - 180 WP_012906750.1 type II toxin-antitoxin system HicA family toxin Toxin
KQH36_RS07555 (KQH36_07570) 1691678..1692406 - 729 WP_004157629.1 two-component system response regulator RstA -
KQH36_RS07560 (KQH36_07575) 1692583..1693047 + 465 WP_004157626.1 hypothetical protein -
KQH36_RS07565 (KQH36_07580) 1693051..1694442 - 1392 WP_004157625.1 amino acid permease -
KQH36_RS07570 (KQH36_07585) 1694650..1695600 - 951 WP_004157624.1 DUF1471 family protein YdgH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1687058..1714580 27522


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-126)


Sequences

Toxin        


Download         Length: 60 a.a.        Molecular weight: 6644.85 Da        Isoelectric Point: 10.9132

>T206006 WP_012906750.1 NZ_CP076594:c1691158-1690979 [Erwinia amylovora]
MDSRNAIAMIEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQAGL

Download         Length: 180 bp

>T206006 NZ_CP102061:4215997-4216299 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14829.76 Da        Isoelectric Point: 4.3623

>AT206006 WP_004157632.1 NZ_CP076594:c1690956-1690546 [Erwinia amylovora]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDNYQDAIESAREAIDAHIELLVEDGEAVPEATSVENWLADPDYVGVVWALVD
VDVTRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRVAADKVIGREKR

Download         Length: 411 bp

>AT206006 NZ_CP102061:4216335-4216676 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6XDK4

Antitoxin

Source ID Structure
AlphaFold DB A0A830ZVY0

References