Detailed information of TA system TA205618

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2700868..2701077
• Replicon: chromosome
• Accession: NZ_CP076401
• Organism: Clostridioides difficile strain S-0253

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: KNZ77_RS12390
• Protein ID: WP_004454815.1
• Coordinates: 2700919..2701077 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	2700868..2701002 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNZ77_RS12375 (2696305)	2696305..2697933	+	1629	WP_004454813.1	aspartate 4-decarboxylase	-
KNZ77_RS12380 (2698054)	2698054..2699049	+	996	WP_003431031.1	asparaginase	-
KNZ77_RS12385 (2699227)	2699227..2699535	-	309	WP_225532604.1	BlaI/MecI/CopY family transcriptional regulator	-
- (2700868)	2700868..2701002	+	135	NuclAT_1	-	Antitoxin
- (2700868)	2700868..2701002	+	135	NuclAT_1	-	Antitoxin
- (2700868)	2700868..2701002	+	135	NuclAT_2	-	Antitoxin
KNZ77_RS12390 (2700919)	2700919..2701077	-	159	WP_004454815.1	hypothetical protein	Toxin
KNZ77_RS12395 (2701371)	2701371..2702741	-	1371	WP_021373596.1	cell wall-binding protein Cwp29	-
KNZ77_RS12400 (2702957)	2702957..2703772	+	816	WP_004454819.1	Arm DNA-binding domain-containing protein	-
KNZ77_RS18725 (2703802)	2703802..2704074	+	273	WP_004454820.1	tyrosine-type recombinase/integrase	-
KNZ77_RS12405 (2704437)	2704437..2704517	-	81	Protein_2371	VOC family protein	-
KNZ77_RS12410 (2704557)	2704557..2705225	-	669	WP_021361938.1	VanZ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T205618 WP_004454815.1 NZ_CP076401:c2701077-2700919 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T205618 NZ_CP101774:29846-30157 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Nucleotide

• Length: 135 bp

>AT205618 NZ_CP076401:2700868-2701002 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin