Detailed information of TA system TA205615

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2458329..2458531
• Replicon: chromosome
• Accession: NZ_CP076401
• Organism: Clostridioides difficile strain S-0253

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: KNZ77_RS11275
• Protein ID: WP_004454589.1
• Coordinates: 2458379..2458531 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2458329..2458458 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNZ77_RS11245 (2453446)	2453446..2453763	+	318	WP_009897465.1	hypothetical protein	-
KNZ77_RS11250 (2453899)	2453899..2454363	+	465	WP_011861514.1	hypothetical protein	-
KNZ77_RS11255 (2454691)	2454691..2454852	-	162	WP_004454578.1	hypothetical protein	-
KNZ77_RS18705 (2454904)	2454904..2455299	-	396	WP_011861515.1	hypothetical protein	-
KNZ77_RS18710 (2455352)	2455352..2455717	-	366	WP_011861516.1	Bro-N domain-containing protein	-
KNZ77_RS11265 (2455837)	2455837..2456025	-	189	WP_021369679.1	virulence factor Srl	-
KNZ77_RS11270 (2457262)	2457262..2458185	-	924	WP_004454587.1	SHOCT domain-containing protein	-
- (2458329)	2458329..2458458	+	130	NuclAT_2	-	Antitoxin
- (2458329)	2458329..2458458	+	130	NuclAT_2	-	Antitoxin
- (2458329)	2458329..2458458	+	130	NuclAT_3	-	Antitoxin
KNZ77_RS11275 (2458379)	2458379..2458531	-	153	WP_004454589.1	hypothetical protein	Toxin
KNZ77_RS11280 (2458805)	2458805..2459128	-	324	WP_009897482.1	DUF6054 family protein	-
KNZ77_RS11285 (2459164)	2459164..2459418	-	255	WP_004454592.1	hypothetical protein	-
KNZ77_RS11290 (2459850)	2459850..2460368	-	519	Protein_2149	RNA-guided endonuclease TnpB family protein	-
KNZ77_RS11295 (2460658)	2460658..2461032	-	375	Protein_2150	BlaI/MecI/CopY family transcriptional regulator	-
KNZ77_RS11300 (2461137)	2461137..2461511	-	375	WP_004454597.1	BlaI/MecI/CopY family transcriptional regulator	-
KNZ77_RS11305 (2462315)	2462315..2462803	+	489	WP_004454599.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
KNZ77_RS11310 (2463164)	2463164..2463362	+	199	Protein_2153	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T205615 WP_004454589.1 NZ_CP076401:c2458531-2458379 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T205615 NZ_CP101771:c4395-4129 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Nucleotide

• Length: 130 bp

>AT205615 NZ_CP076401:2458329-2458458 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin