Detailed information of TA system TA205577

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2001439..2001648
• Replicon: chromosome
• Accession: NZ_CP076377
• Organism: Clostridioides difficile strain S-0352

Toxin (Protein)

• Gene name: CD2517.1
• Uniprot ID: Q182K5
• Locus tag: KNZ78_RS09550
• Protein ID: WP_004454815.1
• Coordinates: 2001490..2001648 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	2001439..2001573 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNZ78_RS09535 (1997066)	1997066..1998694	+	1629	WP_004454813.1	aspartate 4-decarboxylase	-
KNZ78_RS09540 (1998815)	1998815..1999810	+	996	WP_003431031.1	asparaginase	-
KNZ78_RS19700 (1999999)	1999999..2000283	-	285	WP_224213953.1	BlaI/MecI/CopY family transcriptional regulator	-
- (2001439)	2001439..2001573	+	135	NuclAT_1	-	Antitoxin
- (2001439)	2001439..2001573	+	135	NuclAT_1	-	Antitoxin
- (2001439)	2001439..2001573	+	135	NuclAT_2	-	Antitoxin
KNZ78_RS09550 (2001490)	2001490..2001648	-	159	WP_004454815.1	hypothetical protein	Toxin
KNZ78_RS09555 (2002007)	2002007..2003377	-	1371	WP_009897709.1	cell wall-binding protein Cwp29	-
KNZ78_RS19710 (2003813)	2003813..2003899	+	87	WP_231298880.1	hypothetical protein	-
KNZ78_RS09570 (2004429)	2004429..2004701	+	273	WP_004454820.1	tyrosine-type recombinase/integrase	-
KNZ78_RS09575 (2005019)	2005019..2005687	-	669	WP_009897711.1	VanZ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5996.20 Da
• Isoelectric Point: 11.3102

>T205577 WP_004454815.1 NZ_CP076377:c2001648-2001490 [Clostridioides difficile]
MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDLKIRFHKTK

Nucleotide

• Length: 159 bp

>T205577 NZ_CP101752:c4434817-4434542 [Pseudomonas citronellolis]
ATGGCGTTGCAGTGGACGAGCAAGGCCTTGTCCGATCTGGCACGGCTCCATGAGTTTCTCGCTCCGGTGAACCGGTCTGC
GGCGGCCCGCACTGTCCAGCAACTGGTCGCCGCGCCGGCTGTTCTGCTGACCAACCCACGCCTCGGCGAGCGCCTGGATG
AATTCGCGCCGCGTGATGTCCGGCGGCTTCTGGTTGGCCATTATGAGATGCGCTACGAGATAAGGGGCTCCGACATCCAT
CTGCTTCGTGTATGGCATACCCGCGAAGAGCGCTGA

Antitoxin

Nucleotide

• Length: 135 bp

>AT205577 NZ_CP076377:2001439-2001573 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCTGTAGAGTGGAGTTCATAATTTAGAAATTACTTAGTTTTATGGAATCTGATTTTTA
AATCAAATTCCCAACCACTCTTAGTTGCCGCTTTGAGTGGTTTTTTACGCTTTCT

Structures

Toxin

• AlphaFold DB: A0A125V849

Antitoxin