Detailed information of TA system TA205574

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1757305..1757507
• Replicon: chromosome
• Accession: NZ_CP076377
• Organism: Clostridioides difficile strain S-0352

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: KNZ78_RS08420
• Protein ID: WP_004454589.1
• Coordinates: 1757355..1757507 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	1757305..1757434 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNZ78_RS08390 (1752554)	1752554..1752871	+	318	WP_009897465.1	hypothetical protein	-
KNZ78_RS08395 (1753007)	1753007..1753471	+	465	WP_004454576.1	hypothetical protein	-
KNZ78_RS19680 (1753500)	1753500..1753670	-	171	Protein_1680	cell wall-binding repeat-containing protein	-
KNZ78_RS08400 (1753800)	1753800..1753961	-	162	WP_004454578.1	hypothetical protein	-
KNZ78_RS19685 (1754013)	1754013..1754465	-	453	WP_009897470.1	hypothetical protein	-
KNZ78_RS19690 (1754462)	1754462..1754827	-	366	WP_009897471.1	Bro-N domain-containing protein	-
KNZ78_RS08410 (1754947)	1754947..1755099	-	153	WP_009897477.1	hypothetical protein	-
KNZ78_RS08415 (1756238)	1756238..1757161	-	924	WP_021364626.1	SHOCT domain-containing protein	-
- (1757305)	1757305..1757434	+	130	NuclAT_4	-	Antitoxin
- (1757305)	1757305..1757434	+	130	NuclAT_4	-	Antitoxin
- (1757305)	1757305..1757434	+	130	NuclAT_7	-	Antitoxin
KNZ78_RS08420 (1757355)	1757355..1757507	-	153	WP_004454589.1	hypothetical protein	Toxin
KNZ78_RS08425 (1757781)	1757781..1758104	-	324	WP_021364628.1	DUF6054 family protein	-
KNZ78_RS08430 (1758140)	1758140..1758394	-	255	WP_004454592.1	hypothetical protein	-
KNZ78_RS08435 (1758826)	1758826..1759344	-	519	Protein_1689	transposase	-
KNZ78_RS19695 (1759817)	1759817..1760008	-	192	WP_021364351.1	BlaI/MecI/CopY family transcriptional regulator	-
KNZ78_RS08445 (1760113)	1760113..1760490	-	378	WP_021364350.1	BlaI/MecI/CopY family transcriptional regulator	-
KNZ78_RS08450 (1761295)	1761295..1761789	+	495	WP_021364347.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
KNZ78_RS08455 (1762150)	1762150..1762348	+	199	Protein_1693	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T205574 WP_004454589.1 NZ_CP076377:c1757507-1757355 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T205574 NZ_CP101752:c1637296-1636883 [Pseudomonas citronellolis]
ATGCTGAAGTTCATGCTCGATACCAACATCTGCATCTTCACCATCAAGAACAAGCCGCAAGTCGTTCGCGAGGCCTTCAA
CCAGCACCATGGGCAGTTGGCGATCAGTTCGGTGACGCTGATGGAACTGATATACGGCGCCGAGAAGTCCTCCATGCCGG
AACACAACCTGGGCATCGTCGAAGGCTTCGCCGCCCGCCTGGAAGTGCTCGACTACGACGGCAACGCGGCGGCCCACACC
GGGCAACTGCGCGCCGAGCTGGCGCGAATCGGCAAGCCCATCGGCCCCTACGACCAGATGATCGCCGGGCACGCCCGTTC
CCGCGGGCTGATCCTGGTCAGCAACAACCTGCGCGAATTCGAGCGAGTGCCGGGGCTGCGCCTGGAGGACTGGACCGTAG
CCCCATCGCCTTAG

Antitoxin

Nucleotide

• Length: 130 bp

>AT205574 NZ_CP076377:1757305-1757434 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin