Detailed information of TA system TA205570

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 891499..891713
• Replicon: chromosome
• Accession: NZ_CP076377
• Organism: Clostridioides difficile strain S-0352

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: KNZ78_RS04375
• Protein ID: WP_003429855.1
• Coordinates: 891499..891660 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	891580..891713 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNZ78_RS04340 (886686)	886686..886976	+	291	WP_021358995.1	HK97 gp10 family phage protein	-
KNZ78_RS04345 (887086)	887086..887892	+	807	WP_021358994.1	hypothetical protein	-
KNZ78_RS04350 (888241)	888241..888678	+	438	WP_021362375.1	hypothetical protein	-
KNZ78_RS04355 (888671)	888671..888847	+	177	WP_021362376.1	hypothetical protein	-
KNZ78_RS04360 (888849)	888849..890159	+	1311	WP_021368245.1	phage tail sheath family protein	-
KNZ78_RS04365 (890176)	890176..890646	+	471	WP_003429851.1	phage tail tube protein	-
KNZ78_RS04370 (890718)	890718..891158	+	441	WP_003429853.1	phage portal protein	-
KNZ78_RS04375 (891499)	891499..891660	+	162	WP_003429855.1	hypothetical protein	Toxin
- (891580)	891580..891713	-	134	NuclAT_2	-	Antitoxin
- (891580)	891580..891713	-	134	NuclAT_2	-	Antitoxin
- (891580)	891580..891713	-	134	NuclAT_3	-	Antitoxin
- (891580)	891580..891713	-	134	NuclAT_5	-	Antitoxin
- (892058)	892058..892163	-	106	NuclAT_10	-	-
- (892058)	892058..892163	-	106	NuclAT_5	-	-
- (892058)	892058..892163	-	106	NuclAT_5	-	-
- (892058)	892058..892163	-	106	NuclAT_8	-	-
KNZ78_RS04380 (892920)	892920..893093	+	174	WP_018112722.1	hypothetical protein	-
KNZ78_RS04385 (893217)	893217..894044	+	828	WP_021368246.1	hypothetical protein	-
KNZ78_RS04390 (894107)	894107..894283	+	177	WP_009894378.1	hypothetical protein	-
KNZ78_RS04395 (894696)	894696..894899	-	204	WP_021367646.1	helix-turn-helix transcriptional regulator	-
KNZ78_RS19630 (895143)	895143..895946	+	804	WP_021377655.1	ORF6N domain-containing protein	-
KNZ78_RS04410 (896523)	896523..896690	+	168	WP_021367649.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T205570 WP_003429855.1 NZ_CP076377:891499-891660 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T205570 NZ_CP101752:684002-684184 [Pseudomonas citronellolis]
ATGAAGTACAGCGAGTTTCGGCGGTGGTTGAAGTCGCAAGGTGTGCAGTTCGAGCCCGGCAAGGGTTCTCACTTCAAGGT
TTACTTCGGCGACAACCAGACGATCTTCCCGGACCACGGTGCCAAGGAAATGGGCGAAGGGCTGAGGAAGAAAATAATCA
AAGACTTGGGGCTCAAGGATTGA

Antitoxin

Nucleotide

• Length: 134 bp

>AT205570 NZ_CP076377:c891713-891580 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTTGCGGTAGAGTGAAGTTCATAATTAAATGAATCTATTTGAACTTATGGAACTTGATTTT
AAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin