Detailed information of TA system TA205564

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 340194..340409
• Replicon: chromosome
• Accession: NZ_CP076377
• Organism: Clostridioides difficile strain S-0352

Toxin (Protein)

• Gene name: CD2889
• Uniprot ID: Q183X5
• Locus tag: KNZ78_RS01670
• Protein ID: WP_011861731.1
• Coordinates: 340194..340337 (+)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	RCd8
Locus tag	-
Coordinates	340259..340409 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNZ78_RS01645 (335866)	335866..336213	+	348	WP_009898409.1	hypothetical protein	-
KNZ78_RS01650 (336379)	336379..336897	+	519	WP_009898407.1	DUF5706 domain-containing protein	-
KNZ78_RS01655 (336961)	336961..338529	-	1569	WP_009898404.1	hypothetical protein	-
KNZ78_RS01660 (338523)	338523..339044	-	522	WP_009898403.1	ImmA/IrrE family metallo-endopeptidase	-
KNZ78_RS01665 (339746)	339746..339934	-	189	WP_009898401.1	hypothetical protein	-
KNZ78_RS01670 (340194)	340194..340337	+	144	WP_011861731.1	hypothetical protein	Toxin
- (340259)	340259..340409	-	151	NuclAT_1	-	Antitoxin
- (340263)	340263..340409	-	147	NuclAT_0	-	-
- (340263)	340263..340409	-	147	NuclAT_0	-	-
- (340263)	340263..340409	-	147	NuclAT_0	-	-
KNZ78_RS01675 (340844)	340844..341221	+	378	WP_021391346.1	BlaI/MecI/CopY family transcriptional regulator	-
KNZ78_RS01680 (341331)	341331..341714	+	384	WP_015984867.1	BlaI/MecI/CopY family transcriptional regulator	-
KNZ78_RS01685 (341908)	341908..342483	+	576	WP_021360401.1	GntR family transcriptional regulator	-
KNZ78_RS01690 (342509)	342509..342922	+	414	WP_009896035.1	PTS sugar transporter subunit IIA	-
KNZ78_RS01695 (342926)	342926..343669	+	744	WP_003437997.1	BtpA/SgcQ family protein	-
KNZ78_RS01700 (343669)	343669..344139	+	471	WP_003428544.1	PTS sugar transporter subunit IIB	-
KNZ78_RS01705 (344156)	344156..344914	+	759	WP_009888882.1	PTS sugar transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5399.34 Da
• Isoelectric Point: 10.8277

>T205564 WP_011861731.1 NZ_CP076377:340194-340337 [Clostridioides difficile]
MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNKKRH

Nucleotide

• Length: 144 bp

>T205564 NZ_CP101743:c5559980-5559102 [Comamonas sp. C11]
ATGCACGAGGATTTGCTGTCGCTGAAACTGCAGCTGCTGCAGACCGGTTTTCCCGAGCGACTGGTGAAGCTTCCCCAGGG
CGTCCAGGTCGCCTGGCGCGAGGCAGGCGACGCCAGCTCCGACTTCGCGGTGGTGCTGCTGCATGGCATCAGCTCCGGGG
CCGCATCCTGGCTGGATGTGGCGCTGCAGCTGGCTGACAAAGTGCGTGTCCTGGCCTGGGATGCCCCCGGTTATGGCGTG
TCGACGCCGCTGGTCCAATCGGCGCCTGCCGATGCCGACTATGCGCATGCGCTGGCCCAGTCGCTGCTGGTGCTGGGCGT
GCGGCGCTGCATCCTGGTCGGGCACTCGCTGGGCGCCCTGATGGCAGCCAGGCTGGCGCTGATGGCCGCGCCGGGACTGG
TCGAGCAACTGGTGCTCATCAGCCCGGCGGGCGGCTACGGCGCGACGGCCAAGGCCGAGCAGCAGGCCAAGGTGCGCGAG
GGTCGTCTGGCTTCGCTGGCCGAGAAGGGGGTCGCGGGTCTGGCGGCCGTCATCGATCAGCGCCTGGTGTCCTCCGAGGC
TCCTGAAGCCGTGCGTGCATGGGTGCGCTGGAATACGGCACGCATGCAGCCGCAGGGCTATGCGCAGGCGGTCGAGCTGC
TATGCGGCAGCGATCTGGCACAGGCACAGGGCAGGCTGGGCATGCCGGTCGAGGTTTGGGTCGGCGAGCACGATGTGGTG
ACCACACCTGCAGCCTGCAAGTCCTGGTCCGAACTGCTGGGCGCGCACTACGGTACGCTTGCGGCTGCCGGTCATGCTTC
ACCCGTGGAACAACCAATGGAGGTCGCGCATAGGCTGGCATCTTTGTTGAACCAAAGCTATTGCCCCAATACCAGCTGA

Antitoxin

Nucleotide

• Length: 151 bp

>AT205564 NZ_CP076377:c340409-340259 [Clostridioides difficile]
GTAAATTCGTATCAAAAACAAAAAAAGAAACTACAATCTATTAGCCTAGAGTGAAGTTTCATTAACTAAATATTAATGTC
GTTTTTTATTAAACTTGATTTTTACATCAAGCTCAAAGTCACTCCTGCCAGAGTGGCTTTTTACTTTTCTG

Structures

Toxin

• AlphaFold DB: A0A069A3R1

Antitoxin