Detailed information of TA system TA205554

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2563791..2563993
• Replicon: chromosome
• Accession: NZ_CP076376
• Organism: Clostridioides difficile strain S-0942

Toxin (Protein)

• Gene name: CD2299.1
• Uniprot ID: Q185H1
• Locus tag: KNZ76_RS12055
• Protein ID: WP_004454589.1
• Coordinates: 2563841..2563993 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	SQ1641
Locus tag	-
Coordinates	2563791..2563920 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KNZ76_RS12025 (2558851)	2558851..2559168	+	318	WP_004454574.1	hypothetical protein	-
KNZ76_RS12030 (2559418)	2559418..2559768	+	351	WP_021360756.1	hypothetical protein	-
KNZ76_RS12035 (2560097)	2560097..2560258	-	162	WP_004454578.1	hypothetical protein	-
KNZ76_RS19020 (2560310)	2560310..2560762	-	453	WP_021360757.1	hypothetical protein	-
KNZ76_RS19025 (2560759)	2560759..2561124	-	366	WP_021360758.1	BRO family protein	-
KNZ76_RS12045 (2561245)	2561245..2561397	-	153	WP_009897477.1	hypothetical protein	-
KNZ76_RS12050 (2562740)	2562740..2563573	-	834	WP_021360759.1	SHOCT domain-containing protein	-
- (2563791)	2563791..2563920	+	130	NuclAT_1	-	Antitoxin
- (2563791)	2563791..2563920	+	130	NuclAT_1	-	Antitoxin
- (2563791)	2563791..2563920	+	130	NuclAT_1	-	Antitoxin
KNZ76_RS12055 (2563841)	2563841..2563993	-	153	WP_004454589.1	hypothetical protein	Toxin
KNZ76_RS12060 (2564266)	2564266..2564598	-	333	WP_004454591.1	DUF6054 family protein	-
KNZ76_RS12065 (2564625)	2564625..2564879	-	255	WP_004454592.1	hypothetical protein	-
KNZ76_RS12070 (2565319)	2565319..2565837	-	519	Protein_2310	transposase	-
KNZ76_RS12075 (2566127)	2566127..2566502	-	376	Protein_2311	BlaI/MecI/CopY family transcriptional regulator	-
KNZ76_RS12080 (2566607)	2566607..2566984	-	378	WP_011861518.1	BlaI/MecI/CopY family transcriptional regulator	-
KNZ76_RS12085 (2567788)	2567788..2568282	+	495	WP_011861519.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
KNZ76_RS12090 (2568667)	2568667..2568841	+	175	Protein_2314	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.92 Da
• Isoelectric Point: 10.6365

>T205554 WP_004454589.1 NZ_CP076376:c2563993-2563841 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Nucleotide

• Length: 153 bp

>T205554 NZ_CP101729:c9185-8871 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Nucleotide

• Length: 130 bp

>AT205554 NZ_CP076376:2563791-2563920 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A125V725

Antitoxin