Detailed information of TA system TA204222

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 2107129..2107325
• Replicon: chromosome
• Accession: NZ_CP075684
• Organism: Clostridioides difficile strain MT4854

Toxin (Protein)

• Gene name: CD1418.2
• Uniprot ID: D5Q3X5
• Locus tag: KK111_RS09975
• Protein ID: WP_003424190.1
• Coordinates: 2107173..2107325 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	CD630_n00500
Locus tag	-
Coordinates	2107129..2107229 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KK111_RS09960 (2102396)	2102396..2102653	-	258	WP_003424182.1	hypothetical protein	-
KK111_RS09965 (2102640)	2102640..2102873	-	234	WP_003424184.1	hypothetical protein	-
KK111_RS09970 (2103061)	2103061..2103249	+	189	WP_003424185.1	helix-turn-helix transcriptional regulator	-
KK111_RS18565 (2103452)	2103452..2103583	-	132	WP_003424186.1	hypothetical protein	-
- (2107129)	2107129..2107229	+	101	NuclAT_0	-	Antitoxin
- (2107129)	2107129..2107229	+	101	NuclAT_0	-	Antitoxin
- (2107129)	2107129..2107229	+	101	NuclAT_0	-	Antitoxin
KK111_RS09975 (2107173)	2107173..2107325	-	153	WP_003424190.1	hypothetical protein	Toxin
KK111_RS09980 (2107845)	2107845..2108087	+	243	WP_003424191.1	hypothetical protein	-
KK111_RS09985 (2108168)	2108168..2108698	+	531	WP_003424192.1	site-specific integrase	-
KK111_RS09990 (2109243)	2109243..2110088	+	846	WP_003424194.1	DUF4878 domain-containing protein	-
KK111_RS09995 (2110312)	2110312..2110476	-	165	WP_003424195.1	hypothetical protein	-
KK111_RS18420 (2111008)	2111008..2111199	-	192	WP_003424196.1	carboxymuconolactone decarboxylase family protein	-
KK111_RS18425 (2111199)	2111199..2111339	-	141	WP_003424197.1	hypothetical protein	-
KK111_RS10005 (2111599)	2111599..2112240	-	642	WP_003424198.1	SdpI family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5744.78 Da
• Isoelectric Point: 10.6276

>T204222 WP_003424190.1 NZ_CP075684:c2107325-2107173 [Clostridioides difficile]
MDNFLQGILASLVASLIVYLTSKLFKKVKSHSGRSDFSFELEIKFKKNKH

Nucleotide

• Length: 153 bp

>T204222 NZ_CP100670:4720866-4721177 [Salmonella enterica subsp. enterica serovar Mbandaka]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGTTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCCTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Nucleotide

• Length: 101 bp

>AT204222 NZ_CP075684:2107129-2107229 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTAGACGTAGAGTGAAGTTCAAATACTAATGTTTATTCTTTTTGAACTTGATTTCTAGTTC
AAAACTAAAGTCACTCCTGCC

Structures

Toxin

• AlphaFold DB: A0A449XCJ1

Antitoxin