Detailed information of TA system TA200148

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1736879..1737099
• Replicon: chromosome
• Accession: NZ_CP073752
• Organism: Clostridioides difficile strain 2015

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: KFK07_RS08220
• Protein ID: WP_003429855.1
• Coordinates: 1736879..1737040 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1736960..1737099 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFK07_RS08190 (KFK07_08190)	1732736..1733173	+	438	WP_009888839.1	hypothetical protein	-
KFK07_RS08195 (KFK07_08195)	1733166..1733342	+	177	WP_009888840.1	hypothetical protein	-
KFK07_RS08200 (KFK07_08200)	1733343..1734653	+	1311	WP_009893136.1	phage tail sheath family protein	-
KFK07_RS08205 (KFK07_08205)	1734670..1735140	+	471	WP_009888842.1	phage tail tube protein	-
KFK07_RS08210 (KFK07_08210)	1735199..1736026	+	828	WP_009888843.1	hypothetical protein	-
KFK07_RS08215 (KFK07_08215)	1736098..1736538	+	441	WP_003429853.1	phage portal protein	-
KFK07_RS08220 (KFK07_08220)	1736879..1737040	+	162	WP_003429855.1	hypothetical protein	Toxin
-	1736960..1737099	-	140	-	-	Antitoxin
KFK07_RS08225 (KFK07_08225)	1738377..1738565	+	189	WP_003429858.1	hypothetical protein	-
KFK07_RS08230 (KFK07_08230)	1738684..1739550	+	867	WP_009888845.1	BRO family protein	-
KFK07_RS19430	1739603..1739737	+	135	WP_009888846.1	hypothetical protein	-
KFK07_RS08235 (KFK07_08235)	1740415..1740942	+	528	WP_009888847.1	DUF4352 domain-containing protein	-
KFK07_RS08240 (KFK07_08240)	1741080..1741847	+	768	WP_009888848.1	DUF4428 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1703090..1769528	66438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T200148 WP_003429855.1 NZ_CP073752:1736879-1737040 [Clostridioides difficile]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T200148 NZ_CP097716:c4209361-4209047 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCACACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Nucleotide

• Length: 140 bp

>AT200148 NZ_CP073752:c1737099-1736960 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGACAGTAGAGTGAAGTTCATAATTTAAACAATTAGTAATTATTTAAATTTGTGGAACTT
GATTTTAAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin