Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3687484..3688163 Replicon chromosome
Accession NZ_CP072565
Organism Escherichia coli strain WG5::PA8

Toxin (Protein)


Gene name ykfI Uniprot ID S1FHS4
Locus tag J8F86_RS17795 Protein ID WP_000854680.1
Coordinates 3687822..3688163 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EWR7
Locus tag J8F86_RS17790 Protein ID WP_000070396.1
Coordinates 3687484..3687801 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8F86_RS17745 (3682922) 3682922..3683743 + 822 WP_000197388.1 DUF932 domain-containing protein -
J8F86_RS17750 (3683960) 3683960..3684661 + 702 WP_000189411.1 WYL domain-containing protein -
J8F86_RS17755 (3684702) 3684702..3684938 + 237 WP_001144031.1 protein YpjK -
J8F86_RS17760 (3684938) 3684938..3685381 + 444 WP_000649865.1 hypothetical protein -
J8F86_RS17765 (3685404) 3685404..3685871 + 468 WP_001385283.1 protein YkfB -
J8F86_RS17770 (3685948) 3685948..3686187 + 240 WP_000194654.1 DUF905 family protein -
J8F86_RS17775 (3686285) 3686285..3686743 + 459 WP_000211838.1 antirestriction protein -
J8F86_RS17780 (3686759) 3686759..3687235 + 477 WP_000811693.1 RadC family protein -
J8F86_RS17785 (3687244) 3687244..3687465 + 222 WP_000691994.1 DUF987 domain-containing protein -
J8F86_RS17790 (3687484) 3687484..3687801 + 318 WP_000070396.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
J8F86_RS17795 (3687822) 3687822..3688163 + 342 WP_000854680.1 type IV toxin-antitoxin system toxin YkfI Toxin
J8F86_RS17805 (3688735) 3688735..3689988 - 1254 WP_000893272.1 glutamate-5-semialdehyde dehydrogenase -
J8F86_RS17810 (3690000) 3690000..3691103 - 1104 WP_001285288.1 glutamate 5-kinase -
J8F86_RS17815 (3691391) 3691391..3692446 + 1056 WP_045145434.1 phosphoporin PhoE -
J8F86_RS17820 (3692485) 3692485..3692886 - 402 WP_047402959.1 sigma factor-binding protein Crl -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3665353..3688383 23030


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12892.95 Da        Isoelectric Point: 9.6543

>T198091 WP_000854680.1 NZ_CP072565:3687822-3688163 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T198091 NZ_CP096841:713345-713746 [Mycobacterium tuberculosis variant bovis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGGGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11982.62 Da        Isoelectric Point: 7.0270

>AT198091 WP_000070396.1 NZ_CP072565:3687484-3687801 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT198091 NZ_CP096841:713121-713348 [Mycobacterium tuberculosis variant bovis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHS4

Antitoxin

Source ID Structure
AlphaFold DB S1EWR7

References