194142

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1085943..1086469 Replicon chromosome
Accession NZ_CP070482
Organism Escherichia coli strain BTH101

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag JWV29_RS05265 Protein ID WP_000323025.1
Coordinates 1086182..1086469 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag JWV29_RS05260 Protein ID WP_000534858.1
Coordinates 1085943..1086182 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JWV29_RS05205 (1080970) 1080970..1081737 - 768 Protein_1020 exonuclease -
JWV29_RS05210 (1081830) 1081830..1082021 - 192 WP_001083297.1 lysis protein YdfD -
JWV29_RS05215 (1082018) 1082018..1082206 - 189 WP_000854559.1 cell division inhibition protein DicB -
JWV29_RS05220 (1082774) 1082774..1082992 - 219 WP_001171942.1 protein YdfC -
JWV29_RS22460 (1083022) 1083022..1083150 - 129 WP_000344964.1 protein YdfB -
JWV29_RS05225 (1083152) 1083152..1083307 - 156 WP_000379575.1 DUF1391 family protein -
JWV29_RS05230 (1083474) 1083474..1083881 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
JWV29_RS05235 (1083965) 1083965..1084195 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
JWV29_RS05240 (1084179) 1084179..1084457 + 279 Protein_1028 protein YdfX -
JWV29_RS05245 (1084492) 1084492..1084641 + 150 WP_011443592.1 protein YdfW -
JWV29_RS05250 (1085078) 1085078..1085410 - 333 WP_001301033.1 FlxA-like family protein -
JWV29_RS05255 (1085613) 1085613..1085918 - 306 WP_001326990.1 protein YdfV -
JWV29_RS05260 (1085943) 1085943..1086182 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
JWV29_RS05265 (1086182) 1086182..1086469 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
JWV29_RS05270 (1086541) 1086541..1086696 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
JWV29_RS05275 (1086913) 1086913..1087164 + 252 WP_000980994.1 protein Rem -
JWV29_RS05280 (1087231) 1087231..1087509 + 279 WP_012304870.1 hypothetical protein -
JWV29_RS05285 (1087511) 1087511..1088560 + 1050 WP_001265198.1 DUF968 domain-containing protein -
JWV29_RS05290 (1088574) 1088574..1089326 + 753 WP_001047135.1 antitermination protein -
JWV29_RS05295 (1089604) 1089604..1089693 - 90 WP_120795389.1 hypothetical protein -
JWV29_RS05300 (1089748) 1089748..1089960 - 213 WP_000087756.1 cold shock-like protein CspF -
JWV29_RS05305 (1090261) 1090261..1090476 + 216 WP_000066484.1 cold shock-like protein CspB -
JWV29_RS05310 (1090840) 1090840..1091010 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
JWV29_RS05315 (1091230) 1091230..1091445 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1077771..1100813 23042


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T194142 WP_000323025.1 NZ_CP070482:1086182-1086469 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T194142 NZ_CP093369:c2414446-2413124 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT194142 WP_000534858.1 NZ_CP070482:1085943-1086182 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT194142 NZ_CP093369:c2414712-2414446 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References