Detailed information of TA system TA187285

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 4055780..4055993
• Replicon: chromosome
• Accession: NZ_CP068558
• Organism: Clostridioides difficile strain CD9501

Toxin (Protein)

• Gene name: CD2889
• Uniprot ID: Q183X5
• Locus tag: JMW52_RS19620
• Protein ID: WP_011861731.1
• Coordinates: 4055850..4055993 (-)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	RCd12
Locus tag	-
Coordinates	4055780..4055924 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW52_RS19585 (4050931)	4050931..4051098	-	168	WP_077701614.1	Arc family DNA-binding protein	-
JMW52_RS19590 (4051767)	4051767..4051889	-	123	WP_237775269.1	helix-turn-helix domain-containing protein	-
JMW52_RS19595 (4052121)	4052121..4052276	+	156	WP_237775090.1	helix-turn-helix transcriptional regulator	-
JMW52_RS19600 (4052279)	4052279..4052422	+	144	WP_237775091.1	hypothetical protein	-
JMW52_RS20075 (4052536)	4052536..4052667	-	132	WP_016056350.1	hypothetical protein	-
JMW52_RS19605 (4053003)	4053003..4053161	+	159	WP_237775092.1	hypothetical protein	-
JMW52_RS19610 (4053238)	4053238..4054258	+	1021	Protein_3835	site-specific integrase	-
JMW52_RS19615 (4054651)	4054651..4054869	-	219	WP_237775052.1	BlaI/MecI/CopY family transcriptional regulator	-
- (4055780)	4055780..4055924	+	145	NuclAT_1	-	Antitoxin
- (4055780)	4055780..4055924	+	145	NuclAT_1	-	Antitoxin
- (4055780)	4055780..4055924	+	145	NuclAT_1	-	Antitoxin
- (4055780)	4055780..4055928	+	149	NuclAT_3	-	-
JMW52_RS19620 (4055850)	4055850..4055993	-	144	WP_011861731.1	hypothetical protein	Toxin
JMW52_RS19625 (4056251)	4056251..4056439	+	189	WP_009898401.1	hypothetical protein	-
JMW52_RS19630 (4057136)	4057136..4057657	+	522	WP_009898403.1	ImmA/IrrE family metallo-endopeptidase	-
JMW52_RS19635 (4057651)	4057651..4057812	+	162	WP_235699274.1	hypothetical protein	-
JMW52_RS19640 (4057809)	4057809..4058018	+	210	WP_237775053.1	hypothetical protein	-
JMW52_RS19645 (4058117)	4058117..4058413	+	297	WP_237775054.1	hypothetical protein	-
JMW52_RS19650 (4058585)	4058585..4059019	+	435	WP_021435012.1	hypothetical protein	-
JMW52_RS19655 (4059345)	4059345..4059653	-	309	WP_237775056.1	DUF5706 domain-containing protein	-
JMW52_RS19660 (4059956)	4059956..4060303	-	348	WP_009898409.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5399.34 Da
• Isoelectric Point: 10.8277

>T187285 WP_011861731.1 NZ_CP068558:c4055993-4055850 [Clostridioides difficile]
MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNKKRH

Nucleotide

• Length: 144 bp

>T187285 NZ_CP089781:c636923-636510 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Nucleotide

• Length: 145 bp

>AT187285 NZ_CP068558:4055780-4055924 [Clostridioides difficile]
GTAAATTCGTATCAAAACAAAAAAGAAACTACAATCTATTAGCCTAGAGCGAAGTTTCATTAACTAAATATTAATGTCGT
TTTTTATTAAACTTGATTTTTACATCAAGCTCAAAGTCACTCCTGCCAGAGTGGCTTTTTACTTT

Structures

Toxin

• AlphaFold DB: A0A069A3R1

Antitoxin