Detailed information of TA system TA187281

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 4004883..4005096
• Replicon: chromosome
• Accession: NZ_CP068558
• Organism: Clostridioides difficile strain CD9501

Toxin (Protein)

• Gene name: CD2889
• Uniprot ID: Q183X5
• Locus tag: JMW52_RS19160
• Protein ID: WP_011861731.1
• Coordinates: 4004953..4005096 (-)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	RCd12
Locus tag	-
Coordinates	4004883..4005027 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW52_RS19140 (4000465)	4000465..4001340	+	876	WP_009904812.1	LysR family transcriptional regulator	-
JMW52_RS19145 (4001488)	4001488..4002693	-	1206	WP_237775050.1	metallophosphoesterase	-
JMW52_RS19150 (4002677)	4002677..4002877	-	201	WP_237775051.1	hypothetical protein	-
JMW52_RS19155 (4003754)	4003754..4003972	-	219	WP_237775052.1	BlaI/MecI/CopY family transcriptional regulator	-
- (4004883)	4004883..4005027	+	145	NuclAT_0	-	Antitoxin
- (4004883)	4004883..4005027	+	145	NuclAT_0	-	Antitoxin
- (4004883)	4004883..4005027	+	145	NuclAT_0	-	Antitoxin
- (4004883)	4004883..4005031	+	149	NuclAT_2	-	-
JMW52_RS19160 (4004953)	4004953..4005096	-	144	WP_011861731.1	hypothetical protein	Toxin
JMW52_RS19165 (4005353)	4005353..4005541	+	189	WP_009898401.1	hypothetical protein	-
JMW52_RS19170 (4006238)	4006238..4006759	+	522	WP_009898403.1	ImmA/IrrE family metallo-endopeptidase	-
JMW52_RS19175 (4006753)	4006753..4006914	+	162	WP_235699274.1	hypothetical protein	-
JMW52_RS19180 (4006911)	4006911..4007120	+	210	WP_237775053.1	hypothetical protein	-
JMW52_RS19185 (4007219)	4007219..4007515	+	297	WP_237775054.1	hypothetical protein	-
JMW52_RS19190 (4007629)	4007629..4008123	+	495	WP_237775055.1	hypothetical protein	-
JMW52_RS19195 (4008449)	4008449..4008757	-	309	WP_237775056.1	DUF5706 domain-containing protein	-
JMW52_RS19200 (4009270)	4009270..4009407	-	138	WP_237775057.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5399.34 Da
• Isoelectric Point: 10.8277

>T187281 WP_011861731.1 NZ_CP068558:c4005096-4004953 [Clostridioides difficile]
MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNKKRH

Nucleotide

• Length: 144 bp

>T187281 NZ_CP089781:362827-363129 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Nucleotide

• Length: 145 bp

>AT187281 NZ_CP068558:4004883-4005027 [Clostridioides difficile]
GTAAATTCGTATCAAAACAAAAAAGAAACTACAATCTATTAGCCTAGAGCGAAGTTTCATTAACTAAATATTAATGTCGT
TTTTTATTAAACTTGATTTTTACATCAAGCTCAAAGTCACTCCTGCCAGAGTGGCTTTTTACTTT

Structures

Toxin

• AlphaFold DB: A0A069A3R1

Antitoxin