Detailed information of TA system TA187262

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 271699..271895
• Replicon: chromosome
• Accession: NZ_CP068552
• Organism: Clostridioides difficile strain 7009825

Toxin (Protein)

• Gene name: CD1418.2
• Uniprot ID: D5Q3X5
• Locus tag: JMW47_RS01215
• Protein ID: WP_003424190.1
• Coordinates: 271699..271851 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	CD630_n00500
Locus tag	-
Coordinates	271795..271895 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMW47_RS01185	266784..267425	+	642	WP_003424198.1	SdpI family protein	-
JMW47_RS01190	267685..268016	+	332	Protein_237	carboxymuconolactone decarboxylase family protein	-
JMW47_RS01195	268548..268712	+	165	WP_003424195.1	hypothetical protein	-
JMW47_RS01200	268936..269781	-	846	WP_003424194.1	DUF4878 domain-containing protein	-
JMW47_RS01205	270326..270871	-	546	WP_077699404.1	site-specific integrase	-
JMW47_RS01210	270937..271179	-	243	WP_003424191.1	hypothetical protein	-
JMW47_RS01215	271699..271851	+	153	WP_003424190.1	hypothetical protein	Toxin
-	271795..271895	-	101	NuclAT_0	-	Antitoxin
JMW47_RS01220	275775..275963	-	189	WP_003424185.1	helix-turn-helix transcriptional regulator	-
JMW47_RS01225	276151..276384	+	234	WP_003424184.1	hypothetical protein	-
JMW47_RS01230	276371..276628	+	258	WP_003424182.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(40)	-	175631..427678	252047

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5744.78 Da
• Isoelectric Point: 10.6276

>T187262 WP_003424190.1 NZ_CP068552:271699-271851 [Clostridioides difficile]
MDNFLQGILASLVASLIVYLTSKLFKKVKSHSGRSDFSFELEIKFKKNKH

Nucleotide

• Length: 153 bp

>T187262 NZ_CP089780:c2851638-2851219 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Nucleotide

• Length: 101 bp

>AT187262 NZ_CP068552:c271895-271795 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTAGACGTAGAGTGAAGTTCAAATACTAATGTTTATTCTTTTTGAACTTGATTTCTAGTTC
AAAACTAAAGTCACTCCTGCC

Structures

Toxin

• AlphaFold DB: A0A449XCJ1

Antitoxin