Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3456175..3456793 Replicon chromosome
Accession NZ_CP067994
Organism Escherichia coli strain CV601

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JJT18_RS16810 Protein ID WP_001291435.1
Coordinates 3456575..3456793 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JJT18_RS16805 Protein ID WP_000344800.1
Coordinates 3456175..3456549 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJT18_RS16795 3451281..3454430 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
JJT18_RS16800 3454923..3455939 + 1017 WP_010723085.1 IS5-like element IS5 family transposase -
JJT18_RS16805 3456175..3456549 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JJT18_RS16810 3456575..3456793 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JJT18_RS16815 3456965..3457516 + 552 WP_000102564.1 maltose O-acetyltransferase -
JJT18_RS16820 3457632..3458102 + 471 WP_000136192.1 YlaC family protein -
JJT18_RS16825 3458266..3459816 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JJT18_RS16830 3459858..3460211 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JJT18_RS16840 3460590..3460901 + 312 WP_000409911.1 MGMT family protein -
JJT18_RS16845 3460932..3461504 - 573 WP_000779842.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3454959..3455939 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T186404 WP_001291435.1 NZ_CP067994:3456575-3456793 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T186404 NZ_CP089427:327154-327522 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT186404 WP_000344800.1 NZ_CP067994:3456175-3456549 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT186404 NZ_CP089427:326936-327157 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References