Detailed information of TA system TA177200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2306182..2306708
• Replicon: chromosome
• Accession: NZ_CP062895
• Organism: Escherichia coli strain Res13-Lact-ER02-39

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: IMP38_RS11155
• Protein ID: WP_000323025.1
• Coordinates: 2306421..2306708 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: IMP38_RS11150
• Protein ID: WP_000534858.1
• Coordinates: 2306182..2306421 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP38_RS11125	2301516..2301872	+	357	WP_001374839.1	class I SAM-dependent methyltransferase	-
IMP38_RS11130	2302483..2302770	+	288	Protein_2175	hypothetical protein	-
IMP38_RS11135	2303539..2304880	+	1342	Protein_2176	ISNCY family transposase	-
IMP38_RS11140	2305317..2305649	-	333	WP_001317460.1	FlxA-like family protein	-
IMP38_RS11145	2305852..2306157	-	306	WP_001326990.1	hypothetical protein	-
IMP38_RS11150	2306182..2306421	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
IMP38_RS11155	2306421..2306708	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
IMP38_RS11160	2306780..2306935	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
IMP38_RS11165	2307152..2307403	+	252	WP_000980994.1	hypothetical protein	-
IMP38_RS11170	2307470..2307748	+	279	WP_012304870.1	hypothetical protein	-
IMP38_RS11175	2307750..2308799	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
IMP38_RS11180	2308813..2309565	+	753	WP_001047135.1	antitermination protein	-
IMP38_RS11185	2309843..2309932	-	90	WP_120795389.1	hypothetical protein	-
IMP38_RS11190	2309987..2310199	-	213	WP_000087756.1	cold shock-like protein CspF	-
IMP38_RS11195	2310469..2311485	+	1017	WP_001322394.1	IS5-like element IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2289226..2324635	35409
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View CP009789 Escherichia coli K-12 strain ER3413 IS/Tn TA006348 3666255..3667235 0.003 View NC_000913 Escherichia coli str. K-12 substr. MG1655 IS/Tn TA006364 3652182..3653162 0.003	flank	IS/Tn	-	-	2310505..2311485	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T177200 WP_000323025.1 NZ_CP062895:2306421-2306708 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T177200 NZ_CP083274:c2984934-2983612 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT177200 WP_000534858.1 NZ_CP062895:2306182-2306421 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT177200 NZ_CP083274:c2985200-2984934 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8