Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2308093..2308619 Replicon chromosome
Accession NZ_CP062883
Organism Escherichia coli strain Res13-Lact-PEA06-10

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag IMP66_RS11170 Protein ID WP_000323025.1
Coordinates 2308332..2308619 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag IMP66_RS11165 Protein ID WP_000534858.1
Coordinates 2308093..2308332 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP66_RS11140 2303427..2303783 + 357 WP_001374839.1 class I SAM-dependent methyltransferase -
IMP66_RS11145 2304394..2304681 + 288 Protein_2178 hypothetical protein -
IMP66_RS11150 2305450..2306791 + 1342 Protein_2179 ISNCY family transposase -
IMP66_RS11155 2307228..2307560 - 333 WP_001317460.1 FlxA-like family protein -
IMP66_RS11160 2307763..2308068 - 306 WP_001326990.1 hypothetical protein -
IMP66_RS11165 2308093..2308332 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
IMP66_RS11170 2308332..2308619 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
IMP66_RS11175 2308691..2308846 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
IMP66_RS11180 2309063..2309314 + 252 WP_000980994.1 hypothetical protein -
IMP66_RS11185 2309381..2309659 + 279 WP_012304870.1 hypothetical protein -
IMP66_RS11190 2309661..2310710 + 1050 WP_001265198.1 DUF968 domain-containing protein -
IMP66_RS11195 2310724..2311476 + 753 WP_001047135.1 antitermination protein -
IMP66_RS11200 2311754..2311843 - 90 WP_120795389.1 hypothetical protein -
IMP66_RS11205 2311898..2312110 - 213 WP_000087756.1 cold shock-like protein CspF -
IMP66_RS11210 2312380..2313396 + 1017 WP_001322394.1 IS5-like element IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2296169..2329196 33027
flank IS/Tn - - 2312416..2313396 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T177127 WP_000323025.1 NZ_CP062883:2308332-2308619 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T177127 NZ_CP083181:c2179311-2179129 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTTACTATCCCGCATCCTAAAAAGGATTTACCAAGCGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAAGTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT177127 WP_000534858.1 NZ_CP062883:2308093-2308332 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT177127 NZ_CP083181:c2179064-2178660 [Acinetobacter baumannii]
ATGTTGTATCCAATTGCTATCGAGAGAGGTACAGACACCGAGGCCTTTGGTGTCACCGTTCCAGATATTCCAGGATGTTT
TAGCGCAGGCGATACATTAGAGGAAGCTATCGAGAACGTTAAAGAGGCAATTTCTGGCCACTTAGAAATCCTTGCTGAAG
ATGGAGAAGAAATTCCATTAGCATCTGACTTAGCAAAATTTGTAGATGATCCAGATTATAAAGGCATGATCTGGGCTGTT
ACTGAGGTGGATGTTAGTCGTTATCTTGGTAAGCCTGAAAAAATCAACGTAACTTTACCTAGCCGTTTAATTCGCAAGAT
TGATGATAATGTAGGTAAAGATAAAAGATTTAAAACTCGTTCTGCTTTTTTGGCCGCTGGTGCTGAAAAGCTACTACATG
CTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References