Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2306187..2306713 Replicon chromosome
Accession NZ_CP062838
Organism Escherichia coli strain Res13-Lact-PER02-33

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag IMQ20_RS11150 Protein ID WP_000323025.1
Coordinates 2306426..2306713 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag IMQ20_RS11145 Protein ID WP_000534858.1
Coordinates 2306187..2306426 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ20_RS11120 2301521..2301877 + 357 WP_001374839.1 class I SAM-dependent methyltransferase -
IMQ20_RS11125 2302488..2302775 + 288 Protein_2174 hypothetical protein -
IMQ20_RS11130 2303544..2304885 + 1342 Protein_2175 ISNCY family transposase -
IMQ20_RS11135 2305322..2305654 - 333 WP_001317460.1 FlxA-like family protein -
IMQ20_RS11140 2305857..2306162 - 306 WP_001326990.1 hypothetical protein -
IMQ20_RS11145 2306187..2306426 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
IMQ20_RS11150 2306426..2306713 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
IMQ20_RS11155 2306785..2306940 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
IMQ20_RS11160 2307157..2307408 + 252 WP_000980994.1 hypothetical protein -
IMQ20_RS11165 2307475..2307753 + 279 WP_012304870.1 hypothetical protein -
IMQ20_RS11170 2307755..2308804 + 1050 WP_001265198.1 DUF968 domain-containing protein -
IMQ20_RS11175 2308818..2309570 + 753 WP_001047135.1 antitermination protein -
IMQ20_RS11180 2309848..2309937 - 90 WP_120795389.1 hypothetical protein -
IMQ20_RS11185 2309992..2310204 - 213 WP_000087756.1 cold shock-like protein CspF -
IMQ20_RS11190 2310474..2311490 + 1017 WP_001322394.1 IS5-like element IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2277509..2324640 47131
flank IS/Tn - - 2310510..2311490 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T176825 WP_000323025.1 NZ_CP062838:2306426-2306713 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T176825 NZ_CP082967:1842536-1842868 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAAAGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT176825 WP_000534858.1 NZ_CP062838:2306187-2306426 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT176825 NZ_CP082967:1842278-1842535 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References