Detailed information of TA system TA176807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 94516..95141
• Replicon: plasmid unnamednovel_0
• Accession: NZ_CP062837
• Organism: Escherichia coli strain Res13-Lact-PER04-33

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IMQ22_RS24565
• Protein ID: WP_000911317.1
• Coordinates: 94743..95141 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: IMQ22_RS24560
• Protein ID: WP_000450532.1
• Coordinates: 94516..94743 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMQ22_RS24555	89915..94434	-	4520	Protein_100	conjugative transfer relaxase/helicase TraI	-
IMQ22_RS24560	94516..94743	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
IMQ22_RS24565	94743..95141	+	399	WP_000911317.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IMQ22_RS24570	95150..97303	-	2154	WP_195686652.1	type IV conjugative transfer system coupling protein TraD	-
IMQ22_RS24575	97555..98286	-	732	WP_000850430.1	complement resistance protein TraT	-
IMQ22_RS24580	98318..98628	-	311	Protein_105	entry exclusion protein	-
IMQ22_RS24585	98661..99677	+	1017	WP_001322394.1	IS5-like element IS5 family transposase	-
IMQ22_RS24590	99825..100007	-	183	Protein_107	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..141255	141255
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View CP009789 Escherichia coli K-12 strain ER3413 IS/Tn TA006348 3666255..3667235 0.003 View NC_000913 Escherichia coli str. K-12 substr. MG1655 IS/Tn TA006364 3652182..3653162 0.003	flank	IS/Tn	-	-	98697..99677	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.14 Da
• Isoelectric Point: 8.5264

>T176807 WP_000911317.1 NZ_CP062837:94743-95141 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T176807 NZ_CP082954:c733027-732545 [Salmonella enterica subsp. arizonae]
ATGGAAATAAATGTTACTGCACCTGAGCTGCTGAACGAGGAGCATATTCTCCAATCTTTCGATTGCGGAAATGATGTATT
AAACGACTGGCTTCGCCGCCGCGCTATGAAGAATCAGCACCTCAATGCATCCCGTACGTTTGTGATCTGCCTTGAATGCA
CAAAACGCGTAGTAGGGTATTATTCTATTGCGACGGGATCCATAAGCCATATCGATTTAGGTCGCAGCCTGCGTCATAAT
ATGCCCGACCCCGTTCCAGTTGTTTTATTGGGGCGTCTTGCTATTGATAAGTGTACTCAGGGTCATCATTTCGGTAAATG
GCTGCTTAATGATGCTGTAACGCGAGTATCTAACCTGGCAGGGCAGGTGGGGATTAAAGCGATCATGGTGCATGCTATTG
ATGAACAAGCGAAAGAATTCTATGAATATTTTGGCTTTGTTCAGTCCCCCGTTGCACCAAACACATTGTTCTACAAGATT
TAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT176807 WP_000450532.1 NZ_CP062837:94516-94743 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT176807 NZ_CP082954:c733302-733018 [Salmonella enterica subsp. arizonae]
ATGCAAACTACAACCCGCAAATCTATTCGTAATAAACAAATAAATATTCGAGCGACTGATGAGGAGCGCGCAGTAATTGA
CTATGCAGCAAAGTTGGTCAGTAAAAATCGCACTGATTTCATCATAGAGAAAGCTGTGAATGAAGCTCAGAATATCATTC
TGGATCAGCGAGTTTTCGTTCTGGATGATGCTCGTTACCAGGCATTCATTGAACAGCTCGAAGCCCCAGTTCAGAATGTT
GAGGGTCGTCAGCGGCTGATGGATGTAAAACCTGAATGGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5