176789

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2306204..2306730 Replicon chromosome
Accession NZ_CP062832
Organism Escherichia coli strain Res13-Lact-PER04-33

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag IMQ22_RS11140 Protein ID WP_000323025.1
Coordinates 2306443..2306730 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag IMQ22_RS11135 Protein ID WP_000534858.1
Coordinates 2306204..2306443 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ22_RS11110 2301538..2301894 + 357 WP_001374839.1 class I SAM-dependent methyltransferase -
IMQ22_RS11115 2302505..2302792 + 288 Protein_2172 hypothetical protein -
IMQ22_RS11120 2303561..2304902 + 1342 Protein_2173 ISNCY family transposase -
IMQ22_RS11125 2305339..2305671 - 333 WP_001317460.1 FlxA-like family protein -
IMQ22_RS11130 2305874..2306179 - 306 WP_001326990.1 hypothetical protein -
IMQ22_RS11135 2306204..2306443 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
IMQ22_RS11140 2306443..2306730 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
IMQ22_RS11145 2306802..2306957 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
IMQ22_RS11150 2307174..2307425 + 252 WP_000980994.1 hypothetical protein -
IMQ22_RS11155 2307492..2307770 + 279 WP_012304870.1 hypothetical protein -
IMQ22_RS11160 2307772..2308821 + 1050 WP_001265198.1 DUF968 domain-containing protein -
IMQ22_RS11165 2308835..2309587 + 753 WP_001047135.1 antitermination protein -
IMQ22_RS11170 2309865..2309954 - 90 WP_120795389.1 hypothetical protein -
IMQ22_RS11175 2310009..2310221 - 213 WP_000087756.1 cold shock-like protein CspF -
IMQ22_RS11180 2310491..2311507 + 1017 WP_001322394.1 IS5-like element IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2289248..2324657 35409
flank IS/Tn - - 2310527..2311507 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T176789 WP_000323025.1 NZ_CP062832:2306443-2306730 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T176789 NZ_CP082937:c69295-68990 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT176789 WP_000534858.1 NZ_CP062832:2306204-2306443 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT176789 NZ_CP082937:c69515-69297 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References