Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2306204..2306730 Replicon chromosome
Accession NZ_CP062832
Organism Escherichia coli strain Res13-Lact-PER04-33

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag IMQ22_RS11140 Protein ID WP_000323025.1
Coordinates 2306443..2306730 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag IMQ22_RS11135 Protein ID WP_000534858.1
Coordinates 2306204..2306443 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ22_RS11110 2301538..2301894 + 357 WP_001374839.1 class I SAM-dependent methyltransferase -
IMQ22_RS11115 2302505..2302792 + 288 Protein_2172 hypothetical protein -
IMQ22_RS11120 2303561..2304902 + 1342 Protein_2173 ISNCY family transposase -
IMQ22_RS11125 2305339..2305671 - 333 WP_001317460.1 FlxA-like family protein -
IMQ22_RS11130 2305874..2306179 - 306 WP_001326990.1 hypothetical protein -
IMQ22_RS11135 2306204..2306443 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
IMQ22_RS11140 2306443..2306730 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
IMQ22_RS11145 2306802..2306957 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
IMQ22_RS11150 2307174..2307425 + 252 WP_000980994.1 hypothetical protein -
IMQ22_RS11155 2307492..2307770 + 279 WP_012304870.1 hypothetical protein -
IMQ22_RS11160 2307772..2308821 + 1050 WP_001265198.1 DUF968 domain-containing protein -
IMQ22_RS11165 2308835..2309587 + 753 WP_001047135.1 antitermination protein -
IMQ22_RS11170 2309865..2309954 - 90 WP_120795389.1 hypothetical protein -
IMQ22_RS11175 2310009..2310221 - 213 WP_000087756.1 cold shock-like protein CspF -
IMQ22_RS11180 2310491..2311507 + 1017 WP_001322394.1 IS5-like element IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2289248..2324657 35409
flank IS/Tn - - 2310527..2311507 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T176789 WP_000323025.1 NZ_CP062832:2306443-2306730 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T176789 NZ_CP082937:c69295-68990 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT176789 WP_000534858.1 NZ_CP062832:2306204-2306443 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT176789 NZ_CP082937:c69515-69297 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References