Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2306175..2306701 Replicon chromosome
Accession NZ_CP062826
Organism Escherichia coli strain Res13-Lact-PER12-33-A

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag IMQ30_RS11145 Protein ID WP_000323025.1
Coordinates 2306414..2306701 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag IMQ30_RS11140 Protein ID WP_000534858.1
Coordinates 2306175..2306414 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMQ30_RS11115 2301509..2301865 + 357 WP_001374839.1 class I SAM-dependent methyltransferase -
IMQ30_RS11120 2302476..2302763 + 288 Protein_2173 hypothetical protein -
IMQ30_RS11125 2303532..2304873 + 1342 Protein_2174 ISNCY family transposase -
IMQ30_RS11130 2305310..2305642 - 333 WP_001317460.1 FlxA-like family protein -
IMQ30_RS11135 2305845..2306150 - 306 WP_001326990.1 hypothetical protein -
IMQ30_RS11140 2306175..2306414 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
IMQ30_RS11145 2306414..2306701 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
IMQ30_RS11150 2306773..2306928 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
IMQ30_RS11155 2307145..2307396 + 252 WP_000980994.1 hypothetical protein -
IMQ30_RS11160 2307463..2307741 + 279 WP_012304870.1 hypothetical protein -
IMQ30_RS11165 2307743..2308792 + 1050 WP_001265198.1 DUF968 domain-containing protein -
IMQ30_RS11170 2308806..2309558 + 753 WP_001047135.1 antitermination protein -
IMQ30_RS11175 2309836..2309925 - 90 WP_120795389.1 hypothetical protein -
IMQ30_RS11180 2309980..2310192 - 213 WP_000087756.1 cold shock-like protein CspF -
IMQ30_RS11185 2310462..2311478 + 1017 WP_001322394.1 IS5-like element IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2289219..2324628 35409
flank IS/Tn - - 2310498..2311478 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T176753 WP_000323025.1 NZ_CP062826:2306414-2306701 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T176753 NZ_CP082926:c2927004-2926750 [Shewanella inventionis]
ATGAACAAAAAACATTTAAGAACGTTAGCGGCTATTTTTGCTAGACCTGTCAGCGGTTCTATCAAGTGGTCTGATATTGA
AGCGCTGTTTATTGCTCTAGGGGCAGACATAGAGGAACGTGAGGGTTCTCGAATTGGTGTGGTGCTGTTTGGTGAAGTTC
AGGTATATCACCGCCCACATCCTCATAAAGAAACTGACAAAGGCGCAGTGGTAAGCGTCAAAAAATGGTTAGAAAGAAAC
GGGGTAAAAGCATGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT176753 WP_000534858.1 NZ_CP062826:2306175-2306414 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT176753 NZ_CP082926:c2926753-2926412 [Shewanella inventionis]
ATGAAAAATCTAATGATGATCAACGGGGTAAAAGCCTTTATTAATTTTGATCCAGACGCTGAAACATTCCGTGGAGAGTT
CGTTGGGTTAAACGGCGGCGCGGATTTCTACGGTGAAAGCATCAATCAATTGGAAGCCGAGGGCGCGAAGTCATTAAGCG
TTTTTTTAGAGATGTGTCAGGAGCGTGGCATTGAGCCTTATAAAAACTTCTCTGGCAAATTCAACGTTCGGATCTCGCCA
GAGGTTCACGCTCGACTGAATGAAATAGCGCTATCGCAAAGCATTAGTTTGAATGCTGCGGTAGAAAATGCAGTCAATGA
CTACATTGCACATTCAGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References