Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1640802..1641328 Replicon chromosome
Accession NZ_CP062249
Organism Escherichia coli strain AML002_ev01

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag IHN46_RS08040 Protein ID WP_000323025.1
Coordinates 1640802..1641089 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag IHN46_RS08045 Protein ID WP_000534858.1
Coordinates 1641089..1641328 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN46_RS07995 1635826..1636041 - 216 WP_000839590.1 phage lysis protein EssD -
IHN46_RS08000 1636795..1637010 - 216 WP_000066484.1 cold shock-like protein CspB -
IHN46_RS08005 1637311..1637523 + 213 WP_000087756.1 cold shock-like protein CspF -
IHN46_RS08010 1637578..1637667 + 90 WP_120795389.1 hypothetical protein -
IHN46_RS08015 1637945..1638697 - 753 WP_001047135.1 antitermination protein -
IHN46_RS08020 1638711..1639760 - 1050 WP_001393597.1 DUF968 domain-containing protein -
IHN46_RS08025 1639762..1640040 - 279 WP_012304870.1 hypothetical protein -
IHN46_RS08030 1640107..1640358 - 252 WP_000980994.1 hypothetical protein -
IHN46_RS08035 1640575..1640730 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
IHN46_RS08040 1640802..1641089 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
IHN46_RS08045 1641089..1641328 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
IHN46_RS08050 1641353..1641658 + 306 WP_001326990.1 hypothetical protein -
IHN46_RS08055 1641861..1642193 + 333 WP_001301033.1 protein FlxA -
IHN46_RS08060 1642630..1642779 - 150 WP_011443592.1 hypothetical protein -
IHN46_RS08065 1642814..1643092 - 279 Protein_1576 hypothetical protein -
IHN46_RS08070 1643076..1643306 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
IHN46_RS08075 1643390..1643797 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
IHN46_RS08080 1643964..1644119 + 156 WP_000379575.1 DUF1391 family protein -
IHN46_RS08085 1644279..1644497 + 219 WP_001171942.1 hypothetical protein -
IHN46_RS08090 1645065..1645253 + 189 WP_000854559.1 cell division inhibition protein DicB -
IHN46_RS08095 1645250..1645441 + 192 WP_001083297.1 lysis protein YdfD -
IHN46_RS08100 1645534..1646301 + 768 Protein_1583 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1626458..1649371 22913


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T174076 WP_000323025.1 NZ_CP062249:c1641089-1640802 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T174076 NZ_CP080530:c710097-709774 [Mesorhizobium sp. AR02]
ATGAAGGTGATTGAATGGCTTGGAAGCAGCAGAGCGAATGTTCGAGCGTTTCCTGACGATGCGCGGATAGAGGCCGGCTG
GCAGCTCGAACTTGTCCAGCGCGGCGACGATCCCGACGACTGGAAGCCTATGCAGATCGTGGGGGCCGGCGTCAGGGAAA
TCAGAGTCCGGGAGGCATCGGGAGCGTTCCGCGTCATCTATCTGGCAACGCTGGAGGACCGCGTTTTGGTGCTTCATGCG
TTCCAGAAGAAGACACAGGCCACATCCAAAAAAGACCTCGATCTGGCGGCTCAACGGCTAAAACGATGGAAGGCAGAACG
ATGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT174076 WP_000534858.1 NZ_CP062249:c1641328-1641089 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT174076 NZ_CP080530:c709777-709496 [Mesorhizobium sp. AR02]
ATGAACGTAGAAATCTATGAGAACGTCTGGGACGCCCTGGCGGACACCGAACAGGAGGCAGCAAACCTTAAAGCGCGCTC
GGCGCTGCTCTACGAAATCCGTAAGGCAGTCCAGCGTTGGGGCATTCCCCAGGAGGAAGCCGCCAAGCGGCTCGGCCTGA
CGCGCCCTCGTATCAATGATCTGCTGCGCGGTAAGCTCGCCAAGTTCTCGCTTGACGCGCTCGTCAACATCGCCGCCTCC
GCTCACTTGGATATAGAAATCCGGGTGAAGGAGGTTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References