Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1640806..1641332 Replicon chromosome
Accession NZ_CP062247
Organism Escherichia coli strain AML002_ev03

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag IHN48_RS08040 Protein ID WP_000323025.1
Coordinates 1640806..1641093 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag IHN48_RS08045 Protein ID WP_000534858.1
Coordinates 1641093..1641332 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IHN48_RS07995 1635830..1636045 - 216 WP_000839590.1 phage lysis protein EssD -
IHN48_RS08000 1636799..1637014 - 216 WP_000066484.1 cold shock-like protein CspB -
IHN48_RS08005 1637315..1637527 + 213 WP_000087756.1 cold shock-like protein CspF -
IHN48_RS08010 1637582..1637671 + 90 WP_120795389.1 hypothetical protein -
IHN48_RS08015 1637949..1638701 - 753 WP_001047135.1 antitermination protein -
IHN48_RS08020 1638715..1639764 - 1050 WP_001393597.1 DUF968 domain-containing protein -
IHN48_RS08025 1639766..1640044 - 279 WP_012304870.1 hypothetical protein -
IHN48_RS08030 1640111..1640362 - 252 WP_000980994.1 hypothetical protein -
IHN48_RS08035 1640579..1640734 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
IHN48_RS08040 1640806..1641093 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
IHN48_RS08045 1641093..1641332 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
IHN48_RS08050 1641357..1641662 + 306 WP_001326990.1 hypothetical protein -
IHN48_RS08055 1641865..1642197 + 333 WP_001301033.1 protein FlxA -
IHN48_RS08060 1642634..1642783 - 150 WP_011443592.1 hypothetical protein -
IHN48_RS08065 1642818..1643096 - 279 Protein_1576 hypothetical protein -
IHN48_RS08070 1643080..1643310 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
IHN48_RS08075 1643394..1643801 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
IHN48_RS08080 1643968..1644123 + 156 WP_000379575.1 DUF1391 family protein -
IHN48_RS08085 1644283..1644501 + 219 WP_001171942.1 hypothetical protein -
IHN48_RS08090 1645069..1645257 + 189 WP_000854559.1 cell division inhibition protein DicB -
IHN48_RS08095 1645254..1645445 + 192 WP_001083297.1 lysis protein YdfD -
IHN48_RS08100 1645538..1646305 + 768 Protein_1583 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1626462..1649375 22913


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T173990 WP_000323025.1 NZ_CP062247:c1641093-1640806 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T173990 NZ_CP080466:1000397-1000675 [Vibrio cholerae]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT173990 WP_000534858.1 NZ_CP062247:c1641332-1641093 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT173990 NZ_CP080466:1000116-1000400 [Vibrio cholerae]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References