171915

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 14486..15129 Replicon plasmid pEC31-2
Accession NZ_CP060988
Organism Escherichia coli strain EC31

Toxin (Protein)


Gene name vagD Uniprot ID A0A7W3HBH1
Locus tag H9212_RS24315 Protein ID WP_044864680.1
Coordinates 14713..15129 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag H9212_RS24310 Protein ID WP_001261276.1
Coordinates 14486..14716 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9212_RS24285 (9684) 9684..10475 - 792 WP_000016493.1 site-specific integrase -
H9212_RS24290 (10472) 10472..11161 - 690 WP_000796228.1 hypothetical protein -
H9212_RS24295 (11205) 11205..11555 - 351 WP_000493379.1 hypothetical protein -
H9212_RS24300 (11951) 11951..13024 + 1074 WP_077890912.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
H9212_RS24305 (13317) 13317..14231 + 915 WP_052686739.1 Abi family protein -
H9212_RS24310 (14486) 14486..14716 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
H9212_RS24315 (14713) 14713..15129 + 417 WP_044864680.1 type II toxin-antitoxin system VapC family toxin Toxin
H9212_RS24320 (15338) 15338..16318 - 981 WP_000019402.1 IS5-like element IS5 family transposase -
H9212_RS24325 (16519) 16519..19584 - 3066 WP_000002334.1 tetrathionate reductase subunit TtrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / qnrS1 / dfrA14 / fosA3 / blaCTX-M-90 / sitABCD - 1..141542 141542
flank IS/Tn - - 15338..16318 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.64 Da        Isoelectric Point: 8.5403

>T171915 WP_044864680.1 NZ_CP060988:14713-15129 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Download         Length: 417 bp

>T171915 NZ_CP078521:c2082151-2081789 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT171915 WP_001261276.1 NZ_CP060988:14486-14716 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT171915 NZ_CP078521:c2082318-2082148 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3HBH1

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References