Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1629796..1630322 Replicon chromosome
Accession NZ_CP060708
Organism Escherichia coli strain MFDpir

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H9446_RS07980 Protein ID WP_000323025.1
Coordinates 1629796..1630083 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H9446_RS07985 Protein ID WP_000534858.1
Coordinates 1630083..1630322 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9446_RS07935 1624820..1625035 - 216 WP_000839590.1 phage lysis protein EssD -
H9446_RS07940 1625789..1626004 - 216 WP_000066484.1 cold shock-like protein CspB -
H9446_RS07945 1626305..1626517 + 213 WP_000087756.1 cold shock-like protein CspF -
H9446_RS07950 1626572..1626661 + 90 WP_120795389.1 hypothetical protein -
H9446_RS07955 1626939..1627691 - 753 WP_001047135.1 antitermination protein -
H9446_RS07960 1627705..1628754 - 1050 WP_001393597.1 DUF968 domain-containing protein -
H9446_RS07965 1628756..1629034 - 279 WP_012304870.1 hypothetical protein -
H9446_RS07970 1629101..1629352 - 252 WP_000980994.1 hypothetical protein -
H9446_RS07975 1629569..1629724 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
H9446_RS07980 1629796..1630083 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H9446_RS07985 1630083..1630322 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H9446_RS07990 1630347..1630652 + 306 WP_001326990.1 hypothetical protein -
H9446_RS07995 1630855..1631187 + 333 WP_001301033.1 protein FlxA -
H9446_RS08000 1631624..1631773 - 150 WP_011443592.1 hypothetical protein -
H9446_RS08005 1631808..1632086 - 279 Protein_1562 hypothetical protein -
H9446_RS08010 1632070..1632300 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
H9446_RS08015 1632384..1632791 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
H9446_RS08020 1632958..1633113 + 156 WP_000379575.1 DUF1391 family protein -
H9446_RS08025 1633273..1633491 + 219 WP_001171942.1 hypothetical protein -
H9446_RS08030 1634059..1634247 + 189 WP_000854559.1 cell division inhibition protein DicB -
H9446_RS08035 1634244..1634435 + 192 WP_001083297.1 lysis protein YdfD -
H9446_RS08040 1634528..1635295 + 768 Protein_1569 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1599254..1654042 54788


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T170780 WP_000323025.1 NZ_CP060708:c1630083-1629796 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T170780 NZ_CP060708:c1630083-1629796 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT170780 WP_000534858.1 NZ_CP060708:c1630322-1630083 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT170780 NZ_CP060708:c1630322-1630083 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References