Detailed information of TA system TA169051

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): CD-RCd/-
• Location: 1720023..1720243
• Replicon: chromosome
• Accession: NZ_CP059592
• Organism: Clostridioides difficile CD196

Toxin (Protein)

• Gene name: CD0956.2
• Uniprot ID: Q183Z5
• Locus tag: H3006_RS08090
• Protein ID: WP_003429855.1
• Coordinates: 1720023..1720184 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	RCd10
Locus tag	-
Coordinates	1720104..1720243 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3006_RS08060 (1715880)	1715880..1716317	+	438	WP_009888839.1	hypothetical protein	-
H3006_RS08065 (1716310)	1716310..1716486	+	177	WP_009888840.1	hypothetical protein	-
H3006_RS08070 (1716487)	1716487..1717797	+	1311	WP_009893136.1	phage tail sheath family protein	-
H3006_RS08075 (1717814)	1717814..1718284	+	471	WP_009888842.1	phage tail tube protein	-
H3006_RS08080 (1718343)	1718343..1719170	+	828	WP_009888843.1	hypothetical protein	-
H3006_RS08085 (1719242)	1719242..1719682	+	441	WP_003429853.1	phage portal protein	-
H3006_RS08090 (1720023)	1720023..1720184	+	162	WP_003429855.1	hypothetical protein	Toxin
- (1720104)	1720104..1720243	-	140	NuclAT_1	-	Antitoxin
- (1720104)	1720104..1720243	-	140	NuclAT_1	-	Antitoxin
- (1720104)	1720104..1720243	-	140	NuclAT_1	-	Antitoxin
- (1720104)	1720104..1720243	-	140	NuclAT_2	-	Antitoxin
- (1720588)	1720588..1720636	-	49	NuclAT_3	-	-
- (1720588)	1720588..1720636	-	49	NuclAT_3	-	-
- (1720588)	1720588..1720636	-	49	NuclAT_4	-	-
- (1720588)	1720588..1720636	-	49	NuclAT_5	-	-
H3006_RS08095 (1721521)	1721521..1721709	+	189	WP_003429858.1	hypothetical protein	-
H3006_RS08100 (1721828)	1721828..1722694	+	867	WP_009888845.1	BRO family protein	-
H3006_RS18740 (1722747)	1722747..1722881	+	135	WP_009888846.1	hypothetical protein	-
H3006_RS08105 (1723559)	1723559..1724086	+	528	WP_009888847.1	DUF4352 domain-containing protein	-
H3006_RS08110 (1724224)	1724224..1724991	+	768	WP_009888848.1	DUF4428 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6120.42 Da
• Isoelectric Point: 10.8938

>T169051 WP_003429855.1 NZ_CP059592:1720023-1720184 [Clostridioides difficile CD196]
MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFDFKIKFHKFK

Nucleotide

• Length: 162 bp

>T169051 NZ_CP076274:c287101-286823 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Nucleotide

• Length: 140 bp

>AT169051 NZ_CP059592:c1720243-1720104 [Clostridioides difficile CD196]
AAGAAGAACTACAATCTATTTGACAGTAGAGTGAAGTTCATAATTTAAACAATTAGTAATTATTTAAATTTGTGGAACTT
GATTTTAAAATCAAATTCCCAGCCACTTTTACTCTTGCCACGAGTTGAGTGGCTTTTTAC

Structures

Toxin

• AlphaFold DB: A0A069AVN2

Antitoxin