Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2078382..2078908 Replicon chromosome
Accession NZ_CP058948
Organism Escherichia coli strain SY3626C5

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HZ988_RS10120 Protein ID WP_000323025.1
Coordinates 2078382..2078669 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HZ988_RS10125 Protein ID WP_000534858.1
Coordinates 2078669..2078908 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZ988_RS10075 2073406..2073621 - 216 WP_000839590.1 phage lysis protein EssD -
HZ988_RS10080 2074375..2074590 - 216 WP_000066484.1 cold shock-like protein CspB -
HZ988_RS10085 2074891..2075103 + 213 WP_000087756.1 cold shock-like protein CspF -
HZ988_RS10090 2075158..2075247 + 90 WP_120795389.1 hypothetical protein -
HZ988_RS10095 2075525..2076277 - 753 WP_001047135.1 antitermination protein -
HZ988_RS10100 2076291..2077340 - 1050 WP_001265198.1 DUF968 domain-containing protein -
HZ988_RS10105 2077342..2077620 - 279 WP_012304870.1 hypothetical protein -
HZ988_RS10110 2077687..2077938 - 252 WP_000980994.1 hypothetical protein -
HZ988_RS10115 2078155..2078310 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
HZ988_RS10120 2078382..2078669 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HZ988_RS10125 2078669..2078908 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HZ988_RS10130 2078933..2079238 + 306 WP_001326990.1 hypothetical protein -
HZ988_RS10135 2079441..2079773 + 333 WP_001301033.1 protein FlxA -
HZ988_RS10140 2080210..2080359 - 150 WP_011443592.1 hypothetical protein -
HZ988_RS10145 2080394..2080672 - 279 Protein_1988 hypothetical protein -
HZ988_RS10150 2080656..2080886 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
HZ988_RS10155 2080970..2081377 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
HZ988_RS10160 2081544..2081699 + 156 WP_000379575.1 DUF1391 family protein -
HZ988_RS10165 2081859..2082077 + 219 WP_001171942.1 hypothetical protein -
HZ988_RS10170 2082645..2082833 + 189 WP_000854559.1 cell division inhibition protein DicB -
HZ988_RS10175 2082830..2083021 + 192 WP_001083297.1 lysis protein YdfD -
HZ988_RS10180 2083114..2083881 + 768 Protein_1995 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2064038..2085744 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T168003 WP_000323025.1 NZ_CP058948:c2078669-2078382 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T168003 NZ_CP075562:c2000276-2000169 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT168003 WP_000534858.1 NZ_CP058948:c2078908-2078669 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT168003 NZ_CP075562:2000092-2000152 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References