Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1532908..1533434 Replicon chromosome
Accession NZ_CP053605
Organism Escherichia coli strain NEB_Turbo

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HO393_RS07455 Protein ID WP_000323025.1
Coordinates 1532908..1533195 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HO393_RS07460 Protein ID WP_000534858.1
Coordinates 1533195..1533434 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO393_RS07410 1527932..1528147 - 216 WP_000839590.1 phage lysis protein EssD -
HO393_RS07415 1528901..1529116 - 216 WP_000066484.1 cold shock-like protein CspB -
HO393_RS07420 1529417..1529629 + 213 WP_000087756.1 cold shock-like protein CspF -
HO393_RS07425 1529684..1529773 + 90 WP_120795389.1 hypothetical protein -
HO393_RS07430 1530051..1530803 - 753 WP_001047135.1 antitermination protein -
HO393_RS07435 1530817..1531866 - 1050 WP_001265198.1 DUF968 domain-containing protein -
HO393_RS07440 1531868..1532146 - 279 WP_012304870.1 hypothetical protein -
HO393_RS07445 1532213..1532464 - 252 WP_000980994.1 hypothetical protein -
HO393_RS07450 1532681..1532836 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
HO393_RS07455 1532908..1533195 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HO393_RS07460 1533195..1533434 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HO393_RS07465 1533459..1533764 + 306 WP_001326990.1 hypothetical protein -
HO393_RS07470 1533967..1534299 + 333 WP_001301033.1 protein FlxA -
HO393_RS07475 1534736..1534885 - 150 WP_011443592.1 hypothetical protein -
HO393_RS07480 1534920..1535198 - 279 Protein_1460 hypothetical protein -
HO393_RS07485 1535182..1535412 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
HO393_RS07490 1535496..1535903 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
HO393_RS07495 1536070..1536225 + 156 WP_000379575.1 DUF1391 family protein -
HO393_RS07500 1536385..1536603 + 219 WP_001171942.1 hypothetical protein -
HO393_RS07505 1536607..1536771 - 165 WP_014639039.1 hypothetical protein -
HO393_RS07510 1537171..1537359 + 189 WP_000854559.1 cell division inhibition protein DicB -
HO393_RS07515 1537356..1537547 + 192 WP_001083297.1 lysis protein YdfD -
HO393_RS07520 1537640..1538407 + 768 Protein_1468 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1517365..1540270 22905


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T157132 WP_000323025.1 NZ_CP053605:c1533195-1532908 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T157132 NZ_CP069077:366541-366843 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT157132 WP_000534858.1 NZ_CP053605:c1533434-1533195 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT157132 NZ_CP069077:366891-367112 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References