Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1735613..1736139 Replicon chromosome
Accession NZ_CP053604
Organism Escherichia coli strain NEB10-beta

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HO394_RS08485 Protein ID WP_000323025.1
Coordinates 1735613..1735900 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HO394_RS08490 Protein ID WP_000534858.1
Coordinates 1735900..1736139 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO394_RS08440 1730637..1730852 - 216 WP_000839590.1 phage lysis protein EssD -
HO394_RS08445 1731606..1731821 - 216 WP_000066484.1 cold shock-like protein CspB -
HO394_RS08450 1732122..1732334 + 213 WP_000087756.1 cold shock-like protein CspF -
HO394_RS08455 1732389..1732478 + 90 WP_120795389.1 hypothetical protein -
HO394_RS08460 1732756..1733508 - 753 WP_001047135.1 antitermination protein -
HO394_RS08465 1733522..1734571 - 1050 WP_001265198.1 DUF968 domain-containing protein -
HO394_RS08470 1734573..1734851 - 279 WP_012304870.1 hypothetical protein -
HO394_RS08475 1734918..1735169 - 252 WP_000980994.1 hypothetical protein -
HO394_RS08480 1735386..1735541 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
HO394_RS08485 1735613..1735900 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HO394_RS08490 1735900..1736139 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HO394_RS08495 1736164..1736469 + 306 WP_001326990.1 hypothetical protein -
HO394_RS08500 1736672..1737004 + 333 WP_001301033.1 protein FlxA -
HO394_RS08505 1737441..1737590 - 150 WP_011443592.1 hypothetical protein -
HO394_RS08510 1737625..1737903 - 279 Protein_1664 hypothetical protein -
HO394_RS08515 1737887..1738117 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
HO394_RS08520 1738201..1738608 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
HO394_RS08525 1738775..1738930 + 156 WP_000379575.1 DUF1391 family protein -
HO394_RS08530 1739090..1739308 + 219 WP_001171942.1 hypothetical protein -
HO394_RS08535 1739312..1739476 - 165 WP_014639039.1 hypothetical protein -
HO394_RS08540 1739876..1740064 + 189 WP_000854559.1 cell division inhibition protein DicB -
HO394_RS08545 1740061..1740252 + 192 WP_001083297.1 lysis protein YdfD -
HO394_RS08550 1740345..1741112 + 768 Protein_1672 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1721269..1744311 23042


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T157102 WP_000323025.1 NZ_CP053604:c1735900-1735613 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T157102 NZ_CP069076:2190298-2190675 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT157102 WP_000534858.1 NZ_CP053604:c1736139-1735900 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT157102 NZ_CP069076:2190717-2191166 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References