Detailed information of TA system TA157102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1735613..1736139
• Replicon: chromosome
• Accession: NZ_CP053604
• Organism: Escherichia coli strain NEB10-beta

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: HO394_RS08485
• Protein ID: WP_000323025.1
• Coordinates: 1735613..1735900 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: HO394_RS08490
• Protein ID: WP_000534858.1
• Coordinates: 1735900..1736139 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO394_RS08440	1730637..1730852	-	216	WP_000839590.1	phage lysis protein EssD	-
HO394_RS08445	1731606..1731821	-	216	WP_000066484.1	cold shock-like protein CspB	-
HO394_RS08450	1732122..1732334	+	213	WP_000087756.1	cold shock-like protein CspF	-
HO394_RS08455	1732389..1732478	+	90	WP_120795389.1	hypothetical protein	-
HO394_RS08460	1732756..1733508	-	753	WP_001047135.1	antitermination protein	-
HO394_RS08465	1733522..1734571	-	1050	WP_001265198.1	DUF968 domain-containing protein	-
HO394_RS08470	1734573..1734851	-	279	WP_012304870.1	hypothetical protein	-
HO394_RS08475	1734918..1735169	-	252	WP_000980994.1	hypothetical protein	-
HO394_RS08480	1735386..1735541	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
HO394_RS08485	1735613..1735900	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
HO394_RS08490	1735900..1736139	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
HO394_RS08495	1736164..1736469	+	306	WP_001326990.1	hypothetical protein	-
HO394_RS08500	1736672..1737004	+	333	WP_001301033.1	protein FlxA	-
HO394_RS08505	1737441..1737590	-	150	WP_011443592.1	hypothetical protein	-
HO394_RS08510	1737625..1737903	-	279	Protein_1664	hypothetical protein	-
HO394_RS08515	1737887..1738117	-	231	WP_000920568.1	dicB transcriptional regulator DicC	-
HO394_RS08520	1738201..1738608	+	408	WP_000448564.1	DNA-binding transcriptional dual regulator DicA	-
HO394_RS08525	1738775..1738930	+	156	WP_000379575.1	DUF1391 family protein	-
HO394_RS08530	1739090..1739308	+	219	WP_001171942.1	hypothetical protein	-
HO394_RS08535	1739312..1739476	-	165	WP_014639039.1	hypothetical protein	-
HO394_RS08540	1739876..1740064	+	189	WP_000854559.1	cell division inhibition protein DicB	-
HO394_RS08545	1740061..1740252	+	192	WP_001083297.1	lysis protein YdfD	-
HO394_RS08550	1740345..1741112	+	768	Protein_1672	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View CP009789 Escherichia coli K-12 strain ER3413 Prophage TA020026 1611431..1634475 0.002 View NC_007779 Escherichia coli str. K-12 substr. W3110 Prophage TA021438 1632716..1654422 0 View NC_017638 Escherichia coli DH1 Prophage TA027404 1615804..1634890 0.003 View NZ_CP017979 Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126 Prophage TA068425 1534772..1559021 0.002 View NZ_CP025520 Escherichia coli strain DH5alpha Prophage TA090479 488442..510148 0	inside	Prophage	-	-	1721269..1744311	23042

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T157102 WP_000323025.1 NZ_CP053604:c1735900-1735613 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T157102 NZ_CP069076:2190298-2190675 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT157102 WP_000534858.1 NZ_CP053604:c1736139-1735900 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT157102 NZ_CP069076:2190717-2191166 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8