Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2204830..2205356 Replicon chromosome
Accession NZ_CP048439
Organism Escherichia coli strain NBRC 3301

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag GXM24_RS10630 Protein ID WP_000323025.1
Coordinates 2205069..2205356 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag GXM24_RS10625 Protein ID WP_000534858.1
Coordinates 2204830..2205069 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GXM24_RS10565 2199857..2200624 - 768 Protein_2056 exonuclease -
GXM24_RS10570 2200717..2200908 - 192 WP_001083297.1 lysis protein YdfD -
GXM24_RS10575 2200905..2201093 - 189 WP_000854559.1 cell division inhibition protein DicB -
GXM24_RS10585 2201661..2201879 - 219 WP_001171942.1 hypothetical protein -
GXM24_RS10590 2202039..2202194 - 156 WP_000379575.1 DUF1391 family protein -
GXM24_RS10595 2202361..2202768 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
GXM24_RS10600 2202852..2203082 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
GXM24_RS10605 2203066..2203344 + 279 Protein_2063 hypothetical protein -
GXM24_RS10610 2203379..2203528 + 150 WP_011443592.1 hypothetical protein -
GXM24_RS10615 2203965..2204297 - 333 WP_001301033.1 protein FlxA -
GXM24_RS10620 2204500..2204805 - 306 WP_001326990.1 hypothetical protein -
GXM24_RS10625 2204830..2205069 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
GXM24_RS10630 2205069..2205356 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
GXM24_RS10635 2205428..2205583 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
GXM24_RS10640 2205800..2206051 + 252 WP_000980994.1 hypothetical protein -
GXM24_RS10645 2206118..2206396 + 279 WP_012304870.1 hypothetical protein -
GXM24_RS10650 2206398..2207447 + 1050 WP_001265198.1 DUF968 domain-containing protein -
GXM24_RS10655 2207461..2208213 + 753 WP_001047135.1 antitermination protein -
GXM24_RS10660 2208491..2208580 - 90 WP_120795389.1 hypothetical protein -
GXM24_RS10665 2208635..2208847 - 213 WP_000087756.1 cold shock-like protein CspF -
GXM24_RS10670 2209148..2209363 + 216 WP_000066484.1 cold shock-like protein CspB -
GXM24_RS10675 2210117..2210332 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2181110..2235898 54788


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T149472 WP_000323025.1 NZ_CP048439:2205069-2205356 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T149472 NZ_CP064113:c303606-303304 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT149472 WP_000534858.1 NZ_CP048439:2204830-2205069 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT149472 NZ_CP064113:c303297-303010 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCTGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTAGGTTAACAATCAAACCCTCTGGGGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References