Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2240138..2240664 Replicon chromosome
Accession NZ_CP046025
Organism Escherichia coli strain C439

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag GJH08_RS10765 Protein ID WP_000323025.1
Coordinates 2240377..2240664 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag GJH08_RS10760 Protein ID WP_000534858.1
Coordinates 2240138..2240377 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJH08_RS10705 2235165..2235932 - 768 Protein_2089 exonuclease -
GJH08_RS10710 2236025..2236216 - 192 WP_001083297.1 lysis protein YdfD -
GJH08_RS10715 2236213..2236401 - 189 WP_000854559.1 cell division inhibition protein DicB -
GJH08_RS10720 2236969..2237187 - 219 WP_001171942.1 hypothetical protein -
GJH08_RS10725 2237347..2237502 - 156 WP_000379575.1 DUF1391 family protein -
GJH08_RS10730 2237669..2238076 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
GJH08_RS10735 2238160..2238390 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
GJH08_RS10740 2238374..2238652 + 279 Protein_2096 hypothetical protein -
GJH08_RS10745 2238687..2238836 + 150 WP_011443592.1 hypothetical protein -
GJH08_RS10750 2239273..2239605 - 333 WP_001301033.1 protein FlxA -
GJH08_RS10755 2239808..2240113 - 306 WP_001326990.1 hypothetical protein -
GJH08_RS10760 2240138..2240377 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
GJH08_RS10765 2240377..2240664 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
GJH08_RS10770 2240736..2240891 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
GJH08_RS10775 2241108..2241359 + 252 WP_000980994.1 hypothetical protein -
GJH08_RS10780 2241426..2241704 + 279 WP_012304870.1 hypothetical protein -
GJH08_RS10785 2241706..2242755 + 1050 WP_001265198.1 DUF968 domain-containing protein -
GJH08_RS10790 2242769..2243521 + 753 WP_001047135.1 antitermination protein -
GJH08_RS10795 2243799..2243888 - 90 WP_120795389.1 hypothetical protein -
GJH08_RS10800 2243943..2244155 - 213 WP_000087756.1 cold shock-like protein CspF -
GJH08_RS10805 2244456..2244671 + 216 WP_000066484.1 cold shock-like protein CspB -
GJH08_RS10810 2245425..2245640 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2233302..2255008 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T143414 WP_000323025.1 NZ_CP046025:2240377-2240664 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T143414 NZ_CP061081:c3915020-3914769 [Marinomonas arctica]
ATGGATGCTATCAGTTATACCGCCGCTCGAGCTAATCTTGCTAAGACAATGGCTAAGGTCTGTGATGATCATTCTCCAGT
GATCATTACGCGCAAAAGTGAAAGCCCAGTTGTAATGATGTCGCTAGAAGATTATGAATCCATGCAAGAAACTACTTACT
TGCTACGTTCACCAGCGAATGCCAGAGTCTTGTTGGAGTCTATTGCTGAACTGGAAGCAGGTAAGGGTGTTGAAAGAGAG
TTAGTCGAGTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT143414 WP_000534858.1 NZ_CP046025:2240138-2240377 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT143414 NZ_CP061081:c3914772-3914518 [Marinomonas arctica]
GTGAGACTCACTTTTTCAACCGCTGCGTGGGATCAGTATCTTTACTGGCAGAGCACAGATAAGAAAGTATTAAAGCGGAT
AAACTTACTAATCAAAGATATTCAGCGTGAACCTTATGAAGGGATTGGCAAGCCTGAACCTCTAAAGCATGGTCTTGCAG
GTTATTGGTCTCGCCGAATCAATGATGAACACCGAATTGTTTATAAGTATCAAGACGATGCAATTTTGATCGCTCAGTTA
CGATATCACTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References