Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 379021..379652 Replicon chromosome
Accession NZ_CP045941
Organism Shigella flexneri 2a strain AUSMDU00010535

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag GJE14_RS01795 Protein ID WP_001259384.1
Coordinates 379021..379296 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag GJE14_RS01800 Protein ID WP_000593555.1
Coordinates 379293..379652 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE14_RS01780 (374039) 374039..375106 + 1068 WP_000361470.1 HlyD family secretion protein -
GJE14_RS01785 (375103) 375103..377838 + 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
GJE14_RS01790 (377838) 377838..378962 + 1125 WP_001216257.1 ABC transporter permease -
GJE14_RS01795 (379021) 379021..379296 + 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
GJE14_RS01800 (379293) 379293..379652 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GJE14_RS01805 (379772) 379772..380173 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
GJE14_RS01810 (380179) 380179..380985 - 807 WP_000173660.1 nickel import ATP-binding protein NikE -
GJE14_RS01815 (380982) 380982..381746 - 765 WP_001136253.1 nickel import ATP-binding protein NikD -
GJE14_RS01820 (381746) 381746..382579 - 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
GJE14_RS01825 (382576) 382576..383520 - 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 357840..379652 21812


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T143063 WP_001259384.1 NZ_CP045941:379021-379296 [Shigella flexneri 2a]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T143063 NZ_CP060933:3011920-3012027 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT143063 WP_000593555.1 NZ_CP045941:379293-379652 [Shigella flexneri 2a]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT143063 NZ_CP060933:c3011867-3011806 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References