Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 987816..988531 Replicon chromosome
Accession NZ_CP045522
Organism Shigella flexneri strain 5908.2

Toxin (Protein)


Gene name yafO Uniprot ID A0A658YZ27
Locus tag GE167_RS04740 Protein ID WP_001263492.1
Coordinates 988112..988531 (+) Length 140 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag GE167_RS04735 Protein ID WP_000554759.1
Coordinates 987816..988109 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GE167_RS04715 (983455) 983455..983952 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
GE167_RS04720 (984169) 984169..985881 - 1713 Protein_941 flagellar biosynthesis protein FlhA -
GE167_RS04725 (985853) 985853..986638 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
GE167_RS04730 (986709) 986709..987764 + 1056 WP_001226172.1 DNA polymerase IV -
GE167_RS04735 (987816) 987816..988109 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
GE167_RS04740 (988112) 988112..988531 + 420 WP_001263492.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
GE167_RS04745 (988565) 988565..989262 + 698 WP_094106546.1 IS1 family transposase -
GE167_RS04750 (989296) 989296..989748 + 453 WP_001059862.1 GNAT family N-acetyltransferase -
GE167_RS04755 (989994) 989994..991038 + 1045 Protein_948 RNA ligase RtcB family protein -
GE167_RS23790 (991154) 991154..991714 + 561 Protein_949 peptide chain release factor H -
GE167_RS04770 (991771) 991771..993228 - 1458 WP_025759252.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB / gtrB 986709..1049874 63165
flank IS/Tn - - 988759..989262 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 16348.95 Da        Isoelectric Point: 8.0871

>T142024 WP_001263492.1 NZ_CP045522:988112-988531 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRFWVMLPTY

Download         Length: 420 bp

>T142024 NZ_CP060286:c3340369-3340088 [Caproiciproducens sp. 7D4C2]
ATGAAATTGGACATCGTTTGGACGTCTCAGTTCAAGAAGGACTACAAGTTAGCGGTGAAGCGCGGGCTGAAAATGGATTT
GCTGGACAATATCATCCGTCAGCTTTCCAAATGTCAGCCTTTACCCGAGAAAAACCGCGACCACCTTTTGACCGGAAATT
ATGTTGGTTACCGGGAATGTCATATTCAAAGCGACTGGCTGCTTGTGTATCGGATTGAGAATGGCGTTTTGGTTTTAACG
CTGGCCCGCACCGGCTCACATAGTGATTTGTTTGGCGAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT142024 WP_000554759.1 NZ_CP045522:987816-988109 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT142024 NZ_CP060286:c3340641-3340366 [Caproiciproducens sp. 7D4C2]
ATGGCCAATACAACAAATTTCAGCGTCCGCATGGACAGCGATATTAAAAAACAATGCGAAGCGCTTTACAGTGAACTCGG
TATGAATCTCACAACGGCTATCAACGTGTTTTTGCGGCAGTCGCTTCGGTCTGGCGGTTTTCCGTTCGACGTGACGCTCA
ATACGCCGAACGCCGTTACCCTCGCCGCCATGCAGGAAGCGGAGAGGCTTGCCAAAGACCCCAACGCCAAACGCTACAGC
GATGTGGAGGAAGCGTTGAGGGAATTGAAAAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A658YZ27

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References