Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2350901..2351427 Replicon chromosome
Accession NZ_CP044410
Organism Escherichia coli strain ecMN1F

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FNW43_RS11400 Protein ID WP_000323025.1
Coordinates 2350901..2351188 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FNW43_RS11405 Protein ID WP_000534858.1
Coordinates 2351188..2351427 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FNW43_RS11355 2345925..2346140 - 216 WP_000839590.1 phage lysis protein EssD -
FNW43_RS11360 2346894..2347109 - 216 WP_000066484.1 cold shock-like protein CspB -
FNW43_RS11365 2347410..2347622 + 213 WP_000087756.1 cold shock-like protein CspF -
FNW43_RS11370 2347677..2347766 + 90 WP_120795389.1 hypothetical protein -
FNW43_RS11375 2348044..2348796 - 753 WP_001047135.1 antitermination protein -
FNW43_RS11380 2348810..2349859 - 1050 WP_001393597.1 DUF968 domain-containing protein -
FNW43_RS11385 2349861..2350139 - 279 WP_012304870.1 hypothetical protein -
FNW43_RS11390 2350206..2350457 - 252 WP_000980994.1 hypothetical protein -
FNW43_RS11395 2350674..2350829 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FNW43_RS11400 2350901..2351188 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FNW43_RS11405 2351188..2351427 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FNW43_RS11410 2351452..2351757 + 306 WP_001326990.1 hypothetical protein -
FNW43_RS11415 2351960..2352292 + 333 WP_001301033.1 protein FlxA -
FNW43_RS11420 2352729..2352878 - 150 WP_011443592.1 hypothetical protein -
FNW43_RS11425 2352913..2353191 - 279 Protein_2197 hypothetical protein -
FNW43_RS11430 2353175..2353405 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
FNW43_RS11435 2353489..2353896 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
FNW43_RS11440 2354063..2354218 + 156 WP_000379575.1 DUF1391 family protein -
FNW43_RS11445 2354378..2354596 + 219 WP_001171942.1 hypothetical protein -
FNW43_RS11455 2355164..2355352 + 189 WP_000854559.1 cell division inhibition protein DicB -
FNW43_RS11460 2355349..2355540 + 192 WP_001083297.1 lysis protein YdfD -
FNW43_RS11465 2355633..2356400 + 768 Protein_2204 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2320359..2375147 54788


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T140893 WP_000323025.1 NZ_CP044410:c2351188-2350901 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T140893 NZ_CP059497:c1865665-1865549 [Bacillus velezensis]
ATGACTGTTTACGAATCATTAATGATAATGATCAATTTTGGCGGATTGATATTAAATACCGTCTTGTTGATCTTCAATAT
AATGATGATTGTAACGTCAAGCCAAAAGAAAAAATAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT140893 WP_000534858.1 NZ_CP044410:c2351427-2351188 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT140893 NZ_CP059497:1865368-1865554 [Bacillus velezensis]
AATGATACAAATAATTATTTATTGCATTTTGATTTGTGGAAATGCATAAAATAAAAAAAGACCTGGGGTGCGCTAACACC
CACTGGTCATTGTTTGTACAAAAAGCTGCCCATCAAAGGGCTTGCTCGTTGTCAAATCTGCGTAGGCCTAACCCTTCAGG
TGTCCAAACTCAAGGGAAGGTCTATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References