Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3875029..3875660 Replicon chromosome
Accession NZ_CP044158
Organism Shigella flexneri strain AR-0423

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag F4V35_RS20080 Protein ID WP_001259384.1
Coordinates 3875385..3875660 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag F4V35_RS20075 Protein ID WP_000593555.1
Coordinates 3875029..3875388 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V35_RS20050 (3871161) 3871161..3872105 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
F4V35_RS20055 (3872102) 3872102..3872935 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
F4V35_RS20060 (3872935) 3872935..3873699 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
F4V35_RS20065 (3873696) 3873696..3874502 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
F4V35_RS20070 (3874508) 3874508..3874909 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
F4V35_RS20075 (3875029) 3875029..3875388 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
F4V35_RS20080 (3875385) 3875385..3875660 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
F4V35_RS20085 (3875719) 3875719..3876843 - 1125 WP_001216257.1 ABC transporter permease -
F4V35_RS20090 (3876843) 3876843..3879578 - 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
F4V35_RS20095 (3879575) 3879575..3880642 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3875029..3894773 19744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T140419 WP_001259384.1 NZ_CP044158:c3875660-3875385 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T140419 NZ_CP059156:c2522492-2522226 [Staphylococcus aureus]
ATGGTTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTAATTAAAAGTATTGACAGAAATGGTGCGTTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT140419 WP_000593555.1 NZ_CP044158:c3875388-3875029 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT140419 NZ_CP059156:c2522743-2522492 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATAGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTGTAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References