Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1840975..1841606 Replicon chromosome
Accession NZ_CP044155
Organism Shigella flexneri strain AR-0424

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag F4V36_RS09465 Protein ID WP_001259384.1
Coordinates 1841331..1841606 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag F4V36_RS09460 Protein ID WP_000593555.1
Coordinates 1840975..1841334 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V36_RS09435 (1837107) 1837107..1838051 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
F4V36_RS09440 (1838048) 1838048..1838881 + 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
F4V36_RS09445 (1838881) 1838881..1839645 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
F4V36_RS09450 (1839642) 1839642..1840448 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
F4V36_RS09455 (1840454) 1840454..1840855 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
F4V36_RS09460 (1840975) 1840975..1841334 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
F4V36_RS09465 (1841331) 1841331..1841606 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
F4V36_RS09470 (1841665) 1841665..1842789 - 1125 WP_001216257.1 ABC transporter permease -
F4V36_RS09475 (1842789) 1842789..1845524 - 2736 WP_000149186.1 ribosome-associated ATPase/putative transporter RbbA -
F4V36_RS09480 (1845521) 1845521..1846588 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1840975..1862309 21334


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T140378 WP_001259384.1 NZ_CP044155:c1841606-1841331 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T140378 NZ_CP059130:c2142988-2142881 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT140378 WP_000593555.1 NZ_CP044155:c1841334-1840975 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT140378 NZ_CP059130:2143036-2143102 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References