Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2877091..2877722 Replicon chromosome
Accession NZ_CP044152
Organism Shigella flexneri strain AR-0425

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag F4V37_RS14610 Protein ID WP_001259384.1
Coordinates 2877091..2877366 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag F4V37_RS14615 Protein ID WP_000593555.1
Coordinates 2877363..2877722 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V37_RS14595 (2872109) 2872109..2873176 + 1068 WP_000361470.1 HlyD family secretion protein -
F4V37_RS14600 (2873173) 2873173..2875908 + 2736 WP_000149170.1 ribosome-associated ATPase/putative transporter RbbA -
F4V37_RS14605 (2875908) 2875908..2877032 + 1125 WP_001216257.1 ABC transporter permease -
F4V37_RS14610 (2877091) 2877091..2877366 + 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
F4V37_RS14615 (2877363) 2877363..2877722 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
F4V37_RS14620 (2877842) 2877842..2878243 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
F4V37_RS14625 (2878249) 2878249..2879055 - 807 WP_000173660.1 nickel import ATP-binding protein NikE -
F4V37_RS14630 (2879052) 2879052..2879816 - 765 WP_001136253.1 nickel import ATP-binding protein NikD -
F4V37_RS14635 (2879816) 2879816..2880649 - 834 WP_001008973.1 nickel ABC transporter permease subunit NikC -
F4V37_RS14640 (2880646) 2880646..2881590 - 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2856700..2877722 21022


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T140354 WP_001259384.1 NZ_CP044152:2877091-2877366 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T140354 NZ_CP059125:c2812741-2812487 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT140354 WP_000593555.1 NZ_CP044152:2877363-2877722 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT140354 NZ_CP059125:c2812989-2812738 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References