Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yjhX-yjhQ/YjhX-GNAT
Location 2613462..2614276 Replicon chromosome
Accession NZ_CP043406
Organism Escherichia coli strain NMBU-W13E19

Toxin (Protein)


Gene name yjhX Uniprot ID S1PA82
Locus tag FZN32_RS12840 Protein ID WP_001054376.1
Coordinates 2613462..2613719 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name yjhQ Uniprot ID U9Z4B8
Locus tag FZN32_RS12845 Protein ID WP_001309181.1
Coordinates 2613731..2614276 (+) Length 182 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZN32_RS12820 2608619..2609980 - 1362 Protein_2511 MFS transporter -
FZN32_RS12825 2610317..2611333 - 1017 WP_001322394.1 IS5-like element IS5 family transposase -
FZN32_RS12830 2611484..2612470 - 987 WP_001323209.1 hypothetical protein -
FZN32_RS12835 2612840..2613085 + 246 Protein_2514 GNAT family N-acetyltransferase -
FZN32_RS12840 2613462..2613719 + 258 WP_001054376.1 hypothetical protein Toxin
FZN32_RS12845 2613731..2614276 + 546 WP_001309181.1 N-acetyltransferase Antitoxin
FZN32_RS12850 2614332..2615078 + 747 WP_000354251.1 class I SAM-dependent methyltransferase -
FZN32_RS12855 2615247..2615465 + 219 Protein_2518 hypothetical protein -
FZN32_RS12860 2615864..2616985 + 1122 WP_000010829.1 M42 family metallopeptidase -
FZN32_RS12865 2616982..2617260 + 279 WP_000722973.1 PTS sugar transporter subunit IIB -
FZN32_RS12870 2617272..2618585 + 1314 WP_000460843.1 galactitol-specific PTS transporter subunit IIC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2611484..2622500 11016
flank IS/Tn - - 2610317..2611297 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Putative toxin of bacterial toxin-antitoxin pair

(1-85)

Antitoxin

GNAT domain

(18-121)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 9734.29 Da        Isoelectric Point: 11.0090

>T138692 WP_001054376.1 NZ_CP043406:2613462-2613719 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Download         Length: 258 bp

>T138692 NZ_CP058209:3989750-3990127 [Escherichia marmotae]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCTGGCATTTCACTGTGCGATGCGGTGAACTTTATCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCGACTGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCAGGAGGCGAAACGATGA

Antitoxin


Download         Length: 182 a.a.        Molecular weight: 19956.90 Da        Isoelectric Point: 6.3277

>AT138692 WP_001309181.1 NZ_CP043406:2613731-2614276 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Download         Length: 546 bp

>AT138692 NZ_CP058209:3989292-3989660 [Escherichia marmotae]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGTGCACGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTAAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PA82

Antitoxin

Source ID Structure
AlphaFold DB U9Z4B8

References