Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2117201..2117727 Replicon chromosome
Accession NZ_CP043213
Organism Escherichia coli O16:H48 strain CV601gfp

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FYA04_RS10440 Protein ID WP_000323025.1
Coordinates 2117201..2117488 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FYA04_RS10445 Protein ID WP_000534858.1
Coordinates 2117488..2117727 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA04_RS10395 2112225..2112440 - 216 WP_000839590.1 phage lysis protein EssD -
FYA04_RS23085 2112660..2112830 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
FYA04_RS10400 2113194..2113409 - 216 WP_000066484.1 cold shock-like protein CspB -
FYA04_RS10405 2113710..2113922 + 213 WP_000087756.1 cold shock-like protein CspF -
FYA04_RS10410 2113977..2114066 + 90 WP_120795389.1 hypothetical protein -
FYA04_RS10415 2114344..2115096 - 753 WP_001047135.1 antitermination protein -
FYA04_RS10420 2115110..2116159 - 1050 WP_001265198.1 DUF968 domain-containing protein -
FYA04_RS10425 2116161..2116439 - 279 WP_012304870.1 hypothetical protein -
FYA04_RS10430 2116506..2116757 - 252 WP_000980994.1 hypothetical protein -
FYA04_RS10435 2116974..2117129 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FYA04_RS10440 2117201..2117488 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FYA04_RS10445 2117488..2117727 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FYA04_RS10450 2117752..2118057 + 306 WP_001326990.1 hypothetical protein -
FYA04_RS10455 2118260..2118592 + 333 WP_001301033.1 protein FlxA -
FYA04_RS10460 2119029..2119178 - 150 WP_011443592.1 hypothetical protein -
FYA04_RS10465 2119213..2119491 - 279 Protein_2044 hypothetical protein -
FYA04_RS10470 2119475..2119705 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
FYA04_RS10475 2119789..2120196 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
FYA04_RS10480 2120363..2120518 + 156 WP_000379575.1 DUF1391 family protein -
FYA04_RS10485 2120678..2120896 + 219 WP_001171942.1 hypothetical protein -
FYA04_RS10495 2121464..2121652 + 189 WP_000854559.1 cell division inhibition protein DicB -
FYA04_RS10500 2121649..2121840 + 192 WP_001083297.1 lysis protein YdfD -
FYA04_RS10505 2121933..2122700 + 768 Protein_2051 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2102857..2124563 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T137913 WP_000323025.1 NZ_CP043213:c2117488-2117201 [Escherichia coli O16:H48]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T137913 NZ_CP043213:c2117488-2117201 [Escherichia coli O16:H48]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT137913 WP_000534858.1 NZ_CP043213:c2117727-2117488 [Escherichia coli O16:H48]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT137913 NZ_CP043213:c2117727-2117488 [Escherichia coli O16:H48]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References