Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2523777..2524303 Replicon chromosome
Accession NZ_CP043211
Organism Escherichia coli O16:H48 strain PG20180050

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FYA05_RS12175 Protein ID WP_000323025.1
Coordinates 2524016..2524303 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FYA05_RS12170 Protein ID WP_000534858.1
Coordinates 2523777..2524016 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA05_RS12110 2518804..2519571 - 768 Protein_2346 exonuclease -
FYA05_RS12115 2519664..2519855 - 192 WP_001083297.1 lysis protein YdfD -
FYA05_RS12120 2519852..2520040 - 189 WP_000854559.1 cell division inhibition protein DicB -
FYA05_RS12130 2520608..2520826 - 219 WP_001171942.1 hypothetical protein -
FYA05_RS12135 2520986..2521141 - 156 WP_000379575.1 DUF1391 family protein -
FYA05_RS12140 2521308..2521715 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
FYA05_RS12145 2521799..2522029 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
FYA05_RS12150 2522013..2522291 + 279 Protein_2353 hypothetical protein -
FYA05_RS12155 2522326..2522475 + 150 WP_011443592.1 hypothetical protein -
FYA05_RS12160 2522912..2523244 - 333 WP_001301033.1 protein FlxA -
FYA05_RS12165 2523447..2523752 - 306 WP_001326990.1 hypothetical protein -
FYA05_RS12170 2523777..2524016 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FYA05_RS12175 2524016..2524303 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FYA05_RS12180 2524375..2524530 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FYA05_RS12185 2524747..2524998 + 252 WP_000980994.1 hypothetical protein -
FYA05_RS12190 2525065..2525343 + 279 WP_012304870.1 hypothetical protein -
FYA05_RS12195 2525345..2526394 + 1050 WP_001265198.1 DUF968 domain-containing protein -
FYA05_RS12200 2526408..2527160 + 753 WP_001047135.1 antitermination protein -
FYA05_RS12205 2527438..2527527 - 90 WP_120795389.1 hypothetical protein -
FYA05_RS12210 2527582..2527794 - 213 WP_000087756.1 cold shock-like protein CspF -
FYA05_RS12215 2528095..2528310 + 216 WP_000066484.1 cold shock-like protein CspB -
FYA05_RS23165 2528674..2528844 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
FYA05_RS12220 2529064..2529279 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2516941..2538647 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T137876 WP_000323025.1 NZ_CP043211:2524016-2524303 [Escherichia coli O16:H48]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T137876 NZ_CP043211:2524016-2524303 [Escherichia coli O16:H48]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT137876 WP_000534858.1 NZ_CP043211:2523777-2524016 [Escherichia coli O16:H48]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT137876 NZ_CP057099:c4671820-4671578 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References