Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2678592..2679118 Replicon chromosome
Accession NZ_CP043199
Organism Escherichia coli O16:H48 strain PG20180060

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FYA11_RS13055 Protein ID WP_000323025.1
Coordinates 2678592..2678879 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FYA11_RS13060 Protein ID WP_000534858.1
Coordinates 2678879..2679118 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA11_RS13010 2673616..2673831 - 216 WP_000839590.1 phage lysis protein EssD -
FYA11_RS23485 2674051..2674221 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
FYA11_RS13015 2674585..2674800 - 216 WP_000066484.1 cold shock-like protein CspB -
FYA11_RS13020 2675101..2675313 + 213 WP_000087756.1 cold shock-like protein CspF -
FYA11_RS13025 2675368..2675457 + 90 WP_120795389.1 hypothetical protein -
FYA11_RS13030 2675735..2676487 - 753 WP_001047135.1 antitermination protein -
FYA11_RS13035 2676501..2677550 - 1050 WP_001265198.1 DUF968 domain-containing protein -
FYA11_RS13040 2677552..2677830 - 279 WP_012304870.1 hypothetical protein -
FYA11_RS13045 2677897..2678148 - 252 WP_000980994.1 hypothetical protein -
FYA11_RS13050 2678365..2678520 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FYA11_RS13055 2678592..2678879 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FYA11_RS13060 2678879..2679118 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FYA11_RS13065 2679143..2679448 + 306 WP_001326990.1 hypothetical protein -
FYA11_RS13070 2679651..2679983 + 333 WP_001301033.1 protein FlxA -
FYA11_RS13075 2680420..2680569 - 150 WP_011443592.1 hypothetical protein -
FYA11_RS13080 2680604..2680882 - 279 Protein_2535 hypothetical protein -
FYA11_RS13085 2680866..2681096 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
FYA11_RS13090 2681180..2681587 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
FYA11_RS13095 2681754..2681909 + 156 WP_000379575.1 DUF1391 family protein -
FYA11_RS13100 2682069..2682287 + 219 WP_001171942.1 hypothetical protein -
FYA11_RS13110 2682855..2683043 + 189 WP_000854559.1 cell division inhibition protein DicB -
FYA11_RS13115 2683040..2683231 + 192 WP_001083297.1 lysis protein YdfD -
FYA11_RS13120 2683324..2684091 + 768 Protein_2542 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2664248..2685954 21706


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T137672 WP_000323025.1 NZ_CP043199:c2678879-2678592 [Escherichia coli O16:H48]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T137672 NZ_CP043199:c2678879-2678592 [Escherichia coli O16:H48]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT137672 WP_000534858.1 NZ_CP043199:c2679118-2678879 [Escherichia coli O16:H48]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT137672 NZ_CP043199:c2679118-2678879 [Escherichia coli O16:H48]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References