Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3815080..3815698 Replicon chromosome
Accession NZ_CP043185
Organism Escherichia coli O16:H48 strain PG20180171

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FYA18_RS18435 Protein ID WP_001291435.1
Coordinates 3815080..3815298 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FYA18_RS18440 Protein ID WP_000344800.1
Coordinates 3815324..3815698 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FYA18_RS18400 3810369..3810941 + 573 WP_000779842.1 YbaY family lipoprotein -
FYA18_RS18405 3810972..3811283 - 312 WP_000409911.1 MGMT family protein -
FYA18_RS18415 3811662..3812015 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FYA18_RS18420 3812057..3813607 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FYA18_RS18425 3813771..3814241 - 471 WP_000136192.1 YlaC family protein -
FYA18_RS18430 3814357..3814908 - 552 WP_000102564.1 maltose O-acetyltransferase -
FYA18_RS18435 3815080..3815298 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
FYA18_RS18440 3815324..3815698 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FYA18_RS18445 3815934..3816914 - 981 WP_000019403.1 IS5-like element IS5 family transposase -
FYA18_RS18450 3817443..3820592 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3815934..3816914 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T137458 WP_001291435.1 NZ_CP043185:c3815298-3815080 [Escherichia coli O16:H48]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T137458 NZ_CP043185:c3815298-3815080 [Escherichia coli O16:H48]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT137458 WP_000344800.1 NZ_CP043185:c3815698-3815324 [Escherichia coli O16:H48]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT137458 NZ_CP043185:c3815698-3815324 [Escherichia coli O16:H48]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References