Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3575493..3576124 Replicon chromosome
Accession NZ_CP042980
Organism Shigella flexneri Y strain PE577

Toxin (Protein)


Gene name hicA Uniprot ID A0A822PMR8
Locus tag FW259_RS18545 Protein ID WP_001259384.1
Coordinates 3575849..3576124 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag FW259_RS18540 Protein ID WP_000593555.1
Coordinates 3575493..3575852 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FW259_RS18515 3571625..3572569 + 945 WP_000947076.1 nickel ABC transporter permease subunit NikB -
FW259_RS18520 3572566..3573399 + 834 WP_001008974.1 nickel ABC transporter permease subunit NikC -
FW259_RS18525 3573399..3574163 + 765 WP_001136253.1 nickel import ATP-binding protein NikD -
FW259_RS18530 3574160..3574966 + 807 WP_000173660.1 nickel import ATP-binding protein NikE -
FW259_RS18535 3574972..3575373 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
FW259_RS18540 3575493..3575852 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FW259_RS18545 3575849..3576124 - 276 WP_001259384.1 type II toxin-antitoxin system HicA family toxin Toxin
FW259_RS18550 3576183..3577307 - 1125 WP_001216257.1 ABC transporter permease -
FW259_RS18555 3577307..3580042 - 2736 WP_000149173.1 ribosome-associated ATPase/putative transporter RbbA -
FW259_RS18560 3580039..3581106 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3575493..3592590 17097


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-81)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10146.89 Da        Isoelectric Point: 10.3748

>T136850 WP_001259384.1 NZ_CP042980:c3576124-3575849 [Shigella flexneri Y]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Download         Length: 276 bp

>T136850 NZ_CP042980:c3576124-3575849 [Shigella flexneri Y]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAGCCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT136850 WP_000593555.1 NZ_CP042980:c3575852-3575493 [Shigella flexneri Y]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT136850 NZ_CP042980:c3575852-3575493 [Shigella flexneri Y]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PMR8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References