Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2349954..2350480 Replicon chromosome
Accession NZ_CP042844
Organism Escherichia coli strain JME66

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FVE22_RS11455 Protein ID WP_000323025.1
Coordinates 2349954..2350241 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FVE22_RS11460 Protein ID WP_000534858.1
Coordinates 2350241..2350480 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FVE22_RS11410 2344978..2345193 - 216 WP_000839590.1 phage lysis protein EssD -
FVE22_RS11415 2345947..2346162 - 216 WP_000066484.1 cold shock-like protein CspB -
FVE22_RS11420 2346463..2346675 + 213 WP_000087756.1 cold shock-like protein CspF -
FVE22_RS11425 2346730..2346819 + 90 WP_120795389.1 hypothetical protein -
FVE22_RS11430 2347097..2347849 - 753 WP_001047135.1 antitermination protein -
FVE22_RS11435 2347863..2348912 - 1050 WP_001393597.1 DUF968 domain-containing protein -
FVE22_RS11440 2348914..2349192 - 279 WP_012304870.1 hypothetical protein -
FVE22_RS11445 2349259..2349510 - 252 WP_000980994.1 hypothetical protein -
FVE22_RS11450 2349727..2349882 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FVE22_RS11455 2349954..2350241 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FVE22_RS11460 2350241..2350480 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FVE22_RS11465 2350505..2350810 + 306 WP_001326990.1 hypothetical protein -
FVE22_RS11470 2351013..2351345 + 333 WP_001301033.1 protein FlxA -
FVE22_RS11475 2351782..2351931 - 150 WP_011443592.1 hypothetical protein -
FVE22_RS11480 2351966..2352244 - 279 Protein_2205 hypothetical protein -
FVE22_RS11485 2352228..2352458 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
FVE22_RS11490 2352542..2352949 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
FVE22_RS11495 2353116..2353271 + 156 WP_000379575.1 DUF1391 family protein -
FVE22_RS11500 2353431..2353649 + 219 WP_001171942.1 hypothetical protein -
FVE22_RS11510 2354217..2354405 + 189 WP_000854559.1 cell division inhibition protein DicB -
FVE22_RS11515 2354402..2354593 + 192 WP_001083297.1 lysis protein YdfD -
FVE22_RS11520 2354686..2355453 + 768 Protein_2212 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2319412..2374200 54788


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T136134 WP_000323025.1 NZ_CP042844:c2350241-2349954 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T136134 NZ_CP042844:c2350241-2349954 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT136134 WP_000534858.1 NZ_CP042844:c2350480-2350241 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT136134 NZ_CP042844:c2350480-2350241 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References