Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1632802..1633328 Replicon chromosome
Accession NZ_CP040664
Organism Escherichia coli strain KR2009

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag FE384_RS08040 Protein ID WP_000323025.1
Coordinates 1632802..1633089 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag FE384_RS08045 Protein ID WP_000534858.1
Coordinates 1633089..1633328 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE384_RS07995 1627837..1628052 - 216 WP_000839590.1 phage lysis protein EssD -
FE384_RS22480 1628272..1628442 + 171 WP_211180497.1 putative zinc-binding protein YnfU -
FE384_RS08000 1628795..1629010 - 216 WP_000066484.1 cold shock-like protein CspB -
FE384_RS08005 1629311..1629523 + 213 WP_000087756.1 cold shock-like protein CspF -
FE384_RS08010 1629578..1629667 + 90 WP_120795389.1 hypothetical protein -
FE384_RS08015 1629945..1630697 - 753 WP_001047135.1 antitermination protein -
FE384_RS08020 1630711..1631760 - 1050 WP_001265198.1 DUF968 domain-containing protein -
FE384_RS08025 1631762..1632040 - 279 WP_012304870.1 hypothetical protein -
FE384_RS08030 1632107..1632358 - 252 WP_000980994.1 hypothetical protein -
FE384_RS08035 1632575..1632730 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
FE384_RS08040 1632802..1633089 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
FE384_RS08045 1633089..1633328 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
FE384_RS08050 1633353..1633658 + 306 WP_001326990.1 hypothetical protein -
FE384_RS08055 1633861..1634193 + 333 WP_001301033.1 protein FlxA -
FE384_RS08060 1634630..1634779 - 150 WP_011443592.1 hypothetical protein -
FE384_RS08065 1634814..1635092 - 279 Protein_1569 hypothetical protein -
FE384_RS08070 1635076..1635306 - 231 WP_000920568.1 dicB transcriptional regulator DicC -
FE384_RS08075 1635390..1635797 + 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
FE384_RS08080 1635964..1636119 + 156 WP_000379575.1 DUF1391 family protein -
FE384_RS08085 1636279..1636497 + 219 WP_001171942.1 hypothetical protein -
FE384_RS08095 1637065..1637253 + 189 WP_000854559.1 cell division inhibition protein DicB -
FE384_RS08100 1637250..1637441 + 192 WP_001083297.1 lysis protein YdfD -
FE384_RS08105 1637534..1638301 + 768 Protein_1576 exonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1618743..1640164 21421


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T126395 WP_000323025.1 NZ_CP040664:c1633089-1632802 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T126395 NZ_CP040664:c1633089-1632802 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT126395 WP_000534858.1 NZ_CP040664:c1633328-1633089 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT126395 NZ_CP040664:c1633328-1633089 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References