Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | vagCD/VapC-VagC |
Location | 53705..54348 | Replicon | plasmid pEC129_3 |
Accession | NZ_CP038456 | ||
Organism | Escherichia coli strain EC-129 |
Toxin (Protein)
Gene name | vagD | Uniprot ID | A0A7W3HBH1 |
Locus tag | E4Z89_RS25705 | Protein ID | WP_044864680.1 |
Coordinates | 53932..54348 (+) | Length | 139 a.a. |
Antitoxin (Protein)
Gene name | vagC | Uniprot ID | Q84A07 |
Locus tag | E4Z89_RS25700 | Protein ID | WP_001261276.1 |
Coordinates | 53705..53935 (+) | Length | 77 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
E4Z89_RS25670 | 48859..49635 | - | 777 | WP_000015958.1 | site-specific integrase | - |
E4Z89_RS25675 | 49632..50375 | - | 744 | WP_000129823.1 | hypothetical protein | - |
E4Z89_RS25680 | 50426..50776 | - | 351 | WP_000493378.1 | hypothetical protein | - |
E4Z89_RS25690 | 51170..52243 | + | 1074 | WP_077890912.1 | type II toxin-antitoxin system RelB/DinJ family antitoxin | - |
E4Z89_RS25695 | 52536..53450 | + | 915 | WP_052686739.1 | Abi family protein | - |
E4Z89_RS25700 | 53705..53935 | + | 231 | WP_001261276.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
E4Z89_RS25705 | 53932..54348 | + | 417 | WP_044864680.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
E4Z89_RS25710 | 54557..55573 | - | 1017 | WP_001310555.1 | IS5-like element IS5 family transposase | - |
E4Z89_RS25715 | 55738..58803 | - | 3066 | WP_000002334.1 | tetrathionate reductase subunit TtrA | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | tet(A) / ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / dfrA14 | - | 1..67794 | 67794 | |
flank | IS/Tn | - | - | 54557..55537 | 980 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15106.64 Da Isoelectric Point: 8.5403
>T122840 WP_044864680.1 NZ_CP038456:53932-54348 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK
Download Length: 417 bp
>T122840 NZ_CP038456:53932-54348 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACAAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACAAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8721.74 Da Isoelectric Point: 4.3533
>AT122840 WP_001261276.1 NZ_CP038456:53705-53935 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP
Download Length: 231 bp
>AT122840 NZ_CP038456:53705-53935 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGTGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCAGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGAGCCATGA
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGTGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCAGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGAGCCATGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7W3HBH1 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A387K023 |