Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 53705..54348 Replicon plasmid pEC129_3
Accession NZ_CP038456
Organism Escherichia coli strain EC-129

Toxin (Protein)


Gene name vagD Uniprot ID A0A7W3HBH1
Locus tag E4Z89_RS25705 Protein ID WP_044864680.1
Coordinates 53932..54348 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag E4Z89_RS25700 Protein ID WP_001261276.1
Coordinates 53705..53935 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4Z89_RS25670 48859..49635 - 777 WP_000015958.1 site-specific integrase -
E4Z89_RS25675 49632..50375 - 744 WP_000129823.1 hypothetical protein -
E4Z89_RS25680 50426..50776 - 351 WP_000493378.1 hypothetical protein -
E4Z89_RS25690 51170..52243 + 1074 WP_077890912.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
E4Z89_RS25695 52536..53450 + 915 WP_052686739.1 Abi family protein -
E4Z89_RS25700 53705..53935 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
E4Z89_RS25705 53932..54348 + 417 WP_044864680.1 type II toxin-antitoxin system VapC family toxin Toxin
E4Z89_RS25710 54557..55573 - 1017 WP_001310555.1 IS5-like element IS5 family transposase -
E4Z89_RS25715 55738..58803 - 3066 WP_000002334.1 tetrathionate reductase subunit TtrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / dfrA14 - 1..67794 67794
flank IS/Tn - - 54557..55537 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.64 Da        Isoelectric Point: 8.5403

>T122840 WP_044864680.1 NZ_CP038456:53932-54348 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFERVPGLVLEDWVK

Download         Length: 417 bp

>T122840 NZ_CP038456:53932-54348 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGTGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCT
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACCGCCATTGC
CGGACACGCCATCGCGGCGGGCGCCATCCTGGTGACAAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTTCTGG
AAGACTGGGTTAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT122840 WP_001261276.1 NZ_CP038456:53705-53935 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT122840 NZ_CP038456:53705-53935 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGTGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCAGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGACGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3HBH1

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References