Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) CD-RCd/-
Location 2566543..2566745 Replicon chromosome
Accession NZ_CP037840
Organism Clostridioides difficile strain Cd5

Toxin (Protein)


Gene name CD2299.1 Uniprot ID Q185H1
Locus tag E1H28_RS12095 Protein ID WP_004454589.1
Coordinates 2566593..2566745 (-) Length 51 a.a.

Antitoxin (RNA)


Gene name SQ1641
Locus tag -
Coordinates 2566543..2566672 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E1H28_RS12060 (2561653) 2561653..2561970 + 318 WP_009897465.1 hypothetical protein -
E1H28_RS12065 (2562106) 2562106..2562570 + 465 WP_004454576.1 hypothetical protein -
E1H28_RS19750 (2562599) 2562599..2562724 - 126 Protein_2313 hypothetical protein -
E1H28_RS12070 (2562899) 2562899..2563060 - 162 WP_004454578.1 hypothetical protein -
E1H28_RS12075 (2563112) 2563112..2563564 - 453 WP_021359435.1 hypothetical protein -
E1H28_RS12080 (2563561) 2563561..2563926 - 366 WP_016729316.1 Bro-N domain-containing protein -
E1H28_RS12085 (2564046) 2564046..2564198 - 153 WP_009897477.1 hypothetical protein -
E1H28_RS12090 (2565515) 2565515..2566324 - 810 WP_018112791.1 hypothetical protein -
- (2566543) 2566543..2566672 + 130 NuclAT_3 - Antitoxin
- (2566543) 2566543..2566672 + 130 NuclAT_3 - Antitoxin
- (2566543) 2566543..2566672 + 130 NuclAT_5 - Antitoxin
E1H28_RS12095 (2566593) 2566593..2566745 - 153 WP_004454589.1 hypothetical protein Toxin
E1H28_RS12100 (2567019) 2567019..2567342 - 324 WP_009897482.1 DUF6054 family protein -
E1H28_RS12105 (2567378) 2567378..2567632 - 255 WP_004454592.1 hypothetical protein -
E1H28_RS12110 (2568064) 2568064..2568582 - 519 Protein_2322 transposase -
E1H28_RS12115 (2568872) 2568872..2569246 - 375 Protein_2323 BlaI/MecI/CopY family transcriptional regulator -
E1H28_RS12120 (2569351) 2569351..2569725 - 375 WP_004454597.1 BlaI/MecI/CopY family transcriptional regulator -
E1H28_RS12125 (2570529) 2570529..2571017 + 489 WP_004454599.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
E1H28_RS12130 (2571402) 2571402..2571576 + 175 Protein_2326 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.92 Da        Isoelectric Point: 10.6365

>T120191 WP_004454589.1 NZ_CP037840:c2566745-2566593 [Clostridioides difficile]
MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINFKYHRKK

Download         Length: 153 bp

>T120191 NZ_CP037840:c2566745-2566593 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin


Download         Length: 130 bp

>AT120191 NZ_CP037840:2566543-2566672 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A125V725

Antitoxin

Download structure file

References